Effect ofBombyx moriChitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide
| Autor: | Katsuhiko Endo, Hiroyuki Tado, Khondkar Ehteshamul Kabir, Akira Yamanaka, Shuhei Tanaka, Hiroyuki Sugimoto, Daizo Koga |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Aging
media_common.quotation_subject Insect Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Microbiology chemistry.chemical_compound Japan Chitin Bombyx mori Botany Animals Pest Control Biological Molecular Biology media_common biology Chitinases fungi Organic Chemistry Midgut General Medicine Bombyx biology.organism_classification Monochamus alternatus Coleoptera Biopesticide chemistry visual_art Chitinase Microscopy Electron Scanning biology.protein visual_art.visual_art_medium Bark Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 70:219-229 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.70.219 |
| Popis: | Bombyx mori chitinase (Bm-CHI), with a molecular mass of 75 kDa, was investigated on the possibility that it can serve as a biocontrol agent against the adult Japanese pine sawyer (JPS), Monochamus alternatus (Coleoptera: Cerambycidae). Oral ingestion of purified chitinase at concentrations of 3 microM (11.25 microg/50 microl) and 0.3 micoM (1.125 microg/50 microl) caused high mortality in JPS, a significant decrease in bark consumption, and, only in high concentration, a slight reduction of body weight. Fluorescence assays indicated that peritrophic membrane (PM) chitin is degraded by the action of orally ingested Bm-CHI at 3 microM concentration only. Scanning electron micrographs clearly indicated that the beetles that ingested Bm-CHI of the same high concentration had their PM perforated and disrupted, but ultrastructural studies showed that the ingested chitinase did not affect the midgut epithelium. These findings open up the possibility of using insect chitinase as a biopesticidal enzyme. It should have agronomic potential for insect control. |
| Databáze: | OpenAIRE |
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