Comparative analysis of the seminal plasma proteomes of oligoasthenozoospermic and normozoospermic men
| Autor: | Elisa Giacomini, Giuseppe Ricci, Monica Martinelli, Stefania Luppi, Rodolfo C. Garcia, Blendi Ura, Elena Giolo |
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| Přispěvatelé: | Giacomini, Elisa, Ura, Blendi, Giolo, Elena, Luppi, Stefania, Martinelli, Monica, Garcia Rodolfo, C., Ricci, Giuseppe |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Proteome quantitative analysi Semen Biology Bioinformatics Protein expression Mass Spectrometry Male infertility Andrology medicine oligoasthenozoospermia Humans Electrophoresis Gel Two-Dimensional protein expression Relative intensity quantitative analysis Isoelectric focusing Obstetrics and Gynecology two-dimensional protein separation medicine.disease Secretory protein Reproductive Medicine Asthenozoospermia Case-Control Studies Quantitative analysis (chemistry) Developmental Biology |
| Popis: | A comparative proteomic study of oligoasthenozoospermic and normozoospermic seminal plasmas was conducted to establish differences in protein expression. Oligoasthenozoospermia (when semen presents with a low concentration and reduced motility of spermatozoa) is common in male infertility. Two-dimensional protein maps from seminal plasma samples from 10 men with normozoospermia and 10 men with idiopathic oligoasthenozoospermia were obtained by isoelectric focusing followed by sodium dodecyl-sulphate polyacrylamide electrophoresis. Map images were analysed using dedicated software involving normalization, spot-to-spot volume comparison and statistical treatment of the results to establish the significance of differences between normal and oligoasthenozoospermic samples. Six out of 1028 spots showed over 1.5-fold relative intensity differences (P < 0.05, analysis of variance). Four proteins were identified by nano liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry of their tryptic peptides and database searches. Two proteins were more than three-fold under-expressed in oligoasthenozoospermia, namely epididymal secretory protein E1 and galectin-3-binding protein; the other (lipocalin-1 and a prolactin-inducible protein form) were over-expressed. The identity and differential expression of epididymal secretory protein E1 was verified by Western-blotting. The statistically significant differential expression of these four proteins in oligoasthenozoospermia compared with normozoospermia provides a molecular basis for further investigations into the pathogenic mechanisms underlying idiopathic oligoasthenozoospermia. |
| Databáze: | OpenAIRE |
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