Fast, sensitive point of care electrochemical molecular system for point mutation and select agent detection
| Autor: | J. A. MacLeod, Dan Wang, W. C. Dicke, Jared J. Drader, A. C. Nemeth, S. Manalili Wheeler, James C. Hannis, G. B. Collier |
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| Rok vydání: | 2016 |
| Předmět: |
Time Factors
Genotype Yersinia pestis Point-of-Care Systems DNA Mutational Analysis Buccal swab Biomedical Engineering Bioengineering Select agent Computational biology Polymerase Chain Reaction 01 natural sciences Biochemistry Microbiology 03 medical and health sciences 0302 clinical medicine Lab-On-A-Chip Devices Humans Point Mutation Francisella tularensis Point of care Biodefense biology 010401 analytical chemistry Electrochemical Techniques General Chemistry Molecular diagnostics biology.organism_classification DNA extraction 0104 chemical sciences Bacillus anthracis Molecular Diagnostic Techniques Influenza A virus 030220 oncology & carcinogenesis Hemochromatosis |
| Zdroj: | Lab on a Chip. 16:2513-2520 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c5lc01532d |
| Popis: | Point of care molecular diagnostics benefits from a portable battery-operated device capable of performing a fast turnaround using reliable inexpensive cartridges. We describe a prototype device for performing a molecular diagnostics test for clinical and biodefense samples in 16 minutes using a prototype capable of an 8 minute PCR reaction, followed by hybridization and detection on an electrochemical microarray based on the i-STAT® system. We used human buccal swabs for hemochromatosis testing including in-device DNA extraction. Additional clinical and biodefense samples included influenza A and bacterial select agents Bacillus anthracis, Yersinia pestis and Francisella tularensis. |
| Databáze: | OpenAIRE |
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