TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 in Clostridium perfringens
| Autor: | Jennifer Anne Parsons, Julian I. Rood, Trudi L. Bannam, Rodney James Devenish |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Clostridium perfringens Protein Conformation Bacteriophages Transposons and Plasmids Mutant Biology medicine.disease_cause Microbiology Plasmid medicine Translocase Molecular Biology Phylogeny Antibacterial agent Genetics fungi Genetic transfer Walker motifs Genetic Variation DNA-Binding Proteins Complementation Conjugation Genetic biology.protein Fimbriae Proteins Plasmids |
| Zdroj: | Monash University |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | The conjugative tetracycline resistance plasmid pCW3 is the paradigm conjugative plasmid in the anaerobic gram-positive pathogen Clostridium perfringens . Two closely related FtsK/SpoIIIE homologs, TcpA and TcpB, are encoded on pCW3, which is significant since FtsK domains are found in coupling proteins of gram-negative conjugation systems. To develop an understanding of the mechanism of conjugative transfer in C. perfringens , we determined the role of these proteins in the conjugation process. Mutation and complementation analysis was used to show that the tcpA gene was essential for the conjugative transfer of pCW3 and that the tcpB gene was not required for transfer. Furthermore, complementation of a pCW3Δ tcpA mutant with divergent tcpA homologs provided experimental evidence that all of the known conjugative plasmids from C. perfringens use a similar transfer mechanism. Functional genetic analysis of the TcpA protein established the essential role in conjugative transfer of its Walker A and Walker B ATP-binding motifs and its FtsK-like RAAG motif. It is postulated that TcpA is the essential DNA translocase or coupling protein encoded by pCW3 and as such represents a key component of the unique conjugation process in C. perfringens . |
| Databáze: | OpenAIRE |
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