Abnormal expression of TIMP-2, SOD, vimentin and PAI proteins in cloned bovine placentae
| Autor: | D. I. Jin, H. R. Lee, H. R. Kim, Kenji Naruse, C. S. Park, R. X. Han |
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| Rok vydání: | 2008 |
| Předmět: |
Nuclear Transfer Techniques
Cloning Organism Placenta Vimentin Animals Genetically Modified chemistry.chemical_compound Endocrinology Western blot Pregnancy Plasminogen Activator Inhibitor 1 medicine Animals reproductive and urinary physiology Gel electrophoresis Fetus Tissue Inhibitor of Metalloproteinase-2 medicine.diagnostic_test biology Superoxide Dismutase Molecular biology medicine.anatomical_structure chemistry Plasminogen activator inhibitor-1 embryonic structures biology.protein Somatic cell nuclear transfer Matrix Metalloproteinase 2 Animal Science and Zoology Cattle Female Plasminogen activator Biotechnology |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 44(4) |
| ISSN: | 1439-0531 |
| Popis: | Cloned mammals suffer from high rates of placental abnormality and foetal loss during pregnancy. We previously used 2-D gel electrophoresis and mass spectrometry for global proteomic analysis of cloned and normal bovine placentae to identify differential protein expression patterns. Here, we used Western blot analysis to confirm the expression levels of several pregnancy-related proteins putatively identified as being differentially expressed in somatic cell nuclear transfer (SCNT) vs normal bovine placentae. The expression levels of tissue inhibitor of metalloproteinase-2 (TIMP-2), its downstream protein, matrix metalloproteinase-2 (MMP-2), superoxide dismutase (SOD), vimentin and plasminogen activator inhibitor-1 (PAI) were analysed in the placentae of SCNT cloned Korean native cattle that died immediately after birth and in normal placentae obtained by AI. Our results revealed that TIMP-2 and SOD were up-regulated in SCNT placenta compared with normal placenta, whereas MMP-2 levels were comparable in cloned and normal placentae, and vimentin and PAI were significantly down-regulated in SCNT compared with normal placentae. Our results suggest that key proteins of placental development are abnormally expressed in SCNT cloned bovine placentae, probably resulting in abnormal placental function and clonal mortality. |
| Databáze: | OpenAIRE |
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