Efficient Dual siRNA and Drug Delivery Using Engineered Lipoproteoplexes
| Autor: | Liming Yin, Nikita Srivastava, Raymond Chen, Lindsay K. Hill, Priya Katyal, Che Fu Liu, Joseph A. Frezzo, Jin Kim Montclare, P. Douglas Renfrew, Richard Bonneau, Haresh T. More |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal structures Polymers and Plastics Antineoplastic Agents Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences polycyclic compounds Materials Chemistry medicine Humans Doxorubicin Viability assay RNA Small Interfering Glyceraldehyde 3-phosphate dehydrogenase Drug Carriers Gene knockdown biology Chemistry fungi Transfection 021001 nanoscience & nanotechnology Lipids Molecular biology 030104 developmental biology Lipofectamine Drug delivery MCF-7 Cells biology.protein Biophysics Breast cancer cells 0210 nano-technology medicine.drug |
| Zdroj: | Biomacromolecules. 18:2688-2698 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.7b00203 |
| Popis: | An engineered supercharged coiled-coil protein (CSP) and the cationic transfection reagent Lipofectamine 2000 are combined to form a lipoproteoplex for the purpose of dual delivery of siRNA and doxorubicin. CSP, bearing an external positive charge and axial hydrophobic pore, demonstrates the ability to condense siRNA and encapsulate the small-molecule chemotherapeutic, doxorubicin. The lipoproteoplex demonstrates improved doxorubicin loading relative to Lipofectamine 2000. Furthermore, it induces effective transfection of GAPDH (60% knockdown) in MCF-7 breast cancer cells with efficiencies comparing favorably to Lipofectamine 2000. When the lipoproteoplex is loaded with doxorubicin, the improved doxorubicin loading (∼40 μg Dox/mg CSP) results in a substantial decrease in MCF-7 cell viability. |
| Databáze: | OpenAIRE |
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