Lymphoblastoid cell variant in contact-mediated cell spreading
| Autor: | William R. Colquhoun, Joseph A. Sorrentino, Andrew P. Whipple, Albert J.T. Millis |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
chemistry.chemical_classification
Glucosamine Cell signaling Glycoconjugate Monosaccharides Cell Clone (cell biology) Cell Communication Cell Biology Biology In vitro Cell Line Clone Cells Cell biology Microscopy Electron medicine.anatomical_structure Differential interference contrast microscopy chemistry Cell culture Mutation Cell Adhesion medicine Humans Lymphocytes Cell adhesion |
| Zdroj: | Experimental Cell Research. 145:369-379 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(83)90015-0 |
| Popis: | We have isolated a clone of human lymphoblastoid cells that is capable of undergoing the phenomenon of contact-mediated cell spreading in vitro. We have detected this behavior when using both transmission electron microscopy (TEM), and differential interference contrast microscopy. Upon cell-cell contact, cells become loosely adherent and then begin to extend cellular processes that contact other cells and the substrate. We have also selected a variant clone that has lost the capability for cell spreading. The adhesions-defective variant becomes adhesion-positive and appears morphologically identical with the adhesive cells only in response to specific amino sugars. In the presence of those sugars the adhesion response is correlated with a shift in the apparent molecular weight of an iodinatable component. We propose that contact-mediated cell spreading in lymphoblastoid cells is mediated by a non-transferable cell surface-associated glycoconjugate. The synthesis of that glycoconjugate is defective in the non-adhesive clone, unless the cells are grown in glucosamine or mannosamine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|