Preclinical therapeutics ex ovo quail eggs as a biomimetic automation-ready xenograft platform
| Autor: | Atiya Mansoor, Stefano Cairo, Sandra Rugonyi, Charles Keller, Noah E. Berlow, Samuel Rasmussen, Lisa Hudson Price |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
animal structures
Eggs Science Antineoplastic Agents Pharmacology In Vitro Techniques Quail Chorioallantoic Membrane Article Efficacy Mice In vivo Biomimetics biology.animal Animals Humans Chemotherapy Cancer models Drug toxicity Tumor microenvironment Multidisciplinary biology Assay systems Coturnix japonica Hep G2 Cells biology.organism_classification In vitro Chorioallantoic membrane Heterografts Medicine Drug Screening Assays Antitumor |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Preclinical cancer research ranges from in vitro studies that are inexpensive and not necessarily reflective of the tumor microenvironment to mouse studies that are better models but prohibitively expensive at scale. Chorioallantoic membrane (CAM) assays utilizing Japanese quail (Coturnix japonica) are a cost-effective screening method to precede and minimize the scope of murine studies for anti-cancer efficacy and drug toxicity. To increase the throughput of CAM assays we have built and optimized an 11-day platform for processing up to 200 quail eggs per screening to evaluate drug efficacy and drug toxicity caused by a therapeutic. We demonstrate ex ovo concordance with murine in vivo studies, even when the in vitro and in vivo studies diverge, suggesting a role for this quail shell-free CAM xenograft assay in the validation of new anti-cancer agents. |
| Databáze: | OpenAIRE |
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