Deciphering life history transcriptomes in different environments
| Autor: | Subhash Rajpurohit, Cassia Cardoso de Oliveira, Allen G. Gibbs, Shripad Tuljapurkar, Meredith V. Trotter, William J. Etges |
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| Rok vydání: | 2014 |
| Předmět: |
Cactaceae
Microarray Molecular Sequence Data Population Environment Biology Article Neurotransmitter binding Transcriptome Meiosis Gene expression Genetics Animals education Mexico Gene Ecology Evolution Behavior and Systematics Life Cycle Stages education.field_of_study Gene Expression Regulation Developmental Sequence Analysis DNA biology.organism_classification Drosophila mojavensis Larva Drosophila Female |
| Zdroj: | Molecular Ecology. 24:151-179 |
| ISSN: | 0962-1083 |
| DOI: | 10.1111/mec.13017 |
| Popis: | We compared whole transcriptome variation in six preadult stages and seven adult female ages in two populations of cactophilic Drosophila mojavensis reared on two host plants in order to understand how differences in gene expression influence standing life history variation. We used Singular Value Decomposition (SVD) to identify dominant trajectories of life cycle gene expression variation, performed pair-wise comparisons of stage and age differences in gene expression across the life cycle, identified when genes exhibited maximum levels of life cycle gene expression, and assessed population and host cactus effects on gene expression. Life cycle SVD analysis returned four significant components of transcriptional variation, revealing functional enrichment of genes responsible for growth, metabolic function, sensory perception, neural function, translation and aging. Host cactus effects on female gene expression revealed population and stage specific differences, including significant host plant effects on larval metabolism and development, as well as adult neurotransmitter binding and courtship behavior gene expression levels. In 3 - 6 day old virgin females, significant up-regulation of genes associated with meiosis and oogenesis was accompanied by down-regulation of genes associated with somatic maintenance, evidence for a life history tradeoff. The transcriptome of D. mojavensis reared in natural environments throughout its life cycle revealed core developmental transitions and genome wide influences on life history variation in natural populations. |
| Databáze: | OpenAIRE |
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