Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
Autor: | Yoav D. Shaul, Anees Khatib, Balakrishnan Solaimuthu, Arata Hayashi |
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Rok vydání: | 2021 |
Předmět: |
General Chemical Engineering
Breast Neoplasms Mice SCID Malignancy General Biochemistry Genetics and Molecular Biology Metastasis Green fluorescent protein Mice Breast cancer In vivo Mice Inbred NOD Cell Line Tumor medicine Bioluminescence imaging Animals Humans Luciferase Neoplasm Metastasis Luciferases General Immunology and Microbiology business.industry General Neuroscience medicine.disease Disease Models Animal Cancer cell Cancer research Female business |
Zdroj: | Journal of Visualized Experiments. |
ISSN: | 1940-087X |
DOI: | 10.3791/63060-v |
Popis: | Breast cancer is a frequent heterogeneous malignancy and the second leading cause of mortality in women, mainly due to distant organ metastasis. Several animal models have been generated, including the widely used orthotopic mouse models, where cancer cells are injected into the mammary fat pad. However, these models cannot help monitor tumor growth kinetics and metastatic colonization. Cutting-edge tools to monitor cancer cells in real time in mice will significantly advance the understanding of tumor biology. Here, breast cancer cell lines stably expressing luciferase and green fluorescent protein (GFP) were established. Specifically, this technique contains two sequential steps initiated by measuring the luciferase activity in vitro and followed by the implantation of the cancer cells into mammary fat pads of nonobese diabetic-severe combined immunodeficiency (NOD-SCID) mice. After the injection, both the tumor growth and metastatic colonization are monitored in real time by the noninvasive bioluminescence imaging system. Then, the quantification of GFP-expressing metastases in the lungs will be examined by fluorescence microscopy to validate the observed bioluminescence results. This sophisticated system combining luciferase and fluorescence-based detection tools evaluates cancer metastasis in vivo, which has great potential for use in breast cancer therapeutics and disease management. |
Databáze: | OpenAIRE |
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