Unusual DNA structures at the integration site of an HIV provirus
| Autor: | Karen E. Schneider, Miguel A. Gama Sosa, Jessica C. Hall, Gregory C. Lukaszewicz, Ruth M. Ruprecht |
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| Rok vydání: | 1989 |
| Předmět: |
Genes
Viral DNA polymerase II DNA Mutational Analysis Molecular Sequence Data Biophysics Biochemistry chemistry.chemical_compound Plasmid Proviruses Molecular Biology Gene Electrophoresis Agar Gel Genetics Base Sequence biology DNA Superhelical Single-Strand Specific DNA and RNA Endonucleases Nucleic acid sequence Cell Biology Endonucleases Molecular biology Chromatin DNA binding site chemistry DNA Transposable Elements HIV-1 biology.protein Nucleic Acid Conformation In vitro recombination DNA |
| Zdroj: | Biochemical and Biophysical Research Communications. 161:134-142 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(89)91571-4 |
| Popis: | Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites. Site I was located within a 25 bp direct repeat in host DNA 0.6 kB upstream from the 5′ LTR. Site II was mapped 0.2 kB upstream from the vif gene start site. Sequence analysis showed that Site I sequences could assume different unusual DNA structures, whereas sequences at Site II could assume either slipped or H-DNA forms. Unusual DNA structures in host DNA may be associated with active chromatin regions and may favor proviral integration. |
| Databáze: | OpenAIRE |
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