Plasma membrane contributes to the formation of pre-autophagosomal structures
| Autor: | Claudia Puri, Kevin Moreau, Luca Jahreiss, David C. Rubinsztein, Brinda Ravikumar |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Autophagosome
media_common.quotation_subject Blotting Western Autophagy-Related Proteins Models Biological Clathrin Article Cell membrane 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission Phagosomes Autophagy medicine Humans Internalization Sequence Deletion 030304 developmental biology Phagosome media_common 0303 health sciences biology Endoplasmic reticulum Cell Membrane Cell Biology Immunohistochemistry Cell biology medicine.anatomical_structure Autophagosome membrane biology.protein Carrier Proteins 030217 neurology & neurosurgery HeLa Cells Protein Binding |
| Zdroj: | Nature cell biology |
| ISSN: | 1476-4679 1465-7392 |
| DOI: | 10.1038/ncb2078 |
| Popis: | Autophagy is a catabolic process in which lysosomes degrade intracytoplasmic contents transported in double-membraned autophagosomes. Autophagosomes are formed by the elongation and fusion of phagophores, which derive from pre-autophagosomal structures. The membrane origins of autophagosomes are unclear and may involve multiple sources, including the endoplasmic reticulum and mitochondria. Here we show in mammalian cells that the heavy chain of clathrin interacts with Atg16L1 and is involved in the formation of Atg16L1-positive early autophagosome precursors. Atg16L1 associated with clathrin-coated structures, and inhibition of clathrin-mediated internalization decreased the formation of both Atg16L1-positive precursors and mature autophagosomes. We tested and demonstrated that the plasma membrane contributes directly to the formation of early Atg16L1-positive autophagosome precursors. This may be particularly important during periods of increased autophagosome formation, because the plasma membrane may serve as a large membrane reservoir that allows cells periods of autophagosome synthesis at levels many-fold higher than under basal conditions, without compromising other processes. |
| Databáze: | OpenAIRE |
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