Characterization of Begomoviruses Sampled during Severe Epidemics in Tomato Cultivars Carrying the Ty-1 Gene

Autor: Miguel A. Aranda, Jesús Agüero, Covadonga Torre, Michel Peterschmitt, Cristina Gómez-Aix, M. Juárez, Cica Urbino, Yolanda Hernando, Livia Donaire
Přispěvatelé: Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Agence Nationale de la Recherche (France)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 9, p 2614 (2018)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Volume 19
Issue 9
ISSN: 1422-0067
Popis: Tomato yellow leaf curl virus (TYLCV, genus Begomovirus, family Geminiviridae) is a major species that causes a tomato disease for which resistant tomato hybrids (mainly carriers of the Ty-1/Ty-3 gene) are being used widely. We have characterized begomoviruses severely affecting resistant tomato crops in Southeast Spain. Circular DNA was prepared from samples by rolling circle amplification, and sequenced by massive sequencing (2015) or cloning and Sanger sequencing (2016). Thus, 23 complete sequences were determined, all belonging to the TYLCV Israel strain (TYLCV-IL). Massive sequencing also revealed the absence of other geminiviral and beta-satellite sequences. A phylogenetic analysis showed that the Spanish isolates belonged to two groups, one related to early TYLCV-IL isolates in the area (Group 1), and another (Group 2) closely related to El Jadida (Morocco) isolates, suggesting a recent introduction. The most parsimonious evolutionary scenario suggested that the TYLCV isolates of Group 2 are back recombinant isolates derived from TYLCV-IS76, a recombinant virus currently predominating in Moroccan epidemics. Thus, an infectious Group 2 clone (TYLCV-Mu15) was constructed and used in in planta competition assays against TYLCV-IS76. TYLCV-Mu15 predominated in single infections, whereas TYLCV-IS76 did so in mixed infections, providing credibility to a scenario of co-occurrence of both types of isolates.
Research in M.A.A., C.U. and M.P labs was funded by the Ministry of Economy, Industry, and Competitiveness of Spain, grant number PCIN-2017-055, and Agence Nationale de la Recherche of France, respectively, both within the ERANET-ARIMNet2 (ref. 302) program. J.A. and C.G.-A. were recipients of grants PTQ-13-05882 and PTQ-15-07646, respectively, from the Torres-Quevedo program (Ministry of Economy, Industry and Competitiveness; Spain), and C.T. of fellowship DI-14-06825 from the Industrial Doctoral program (Ministry of Economy, Industry, and Competitiveness, Spain).
Databáze: OpenAIRE
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