Interferon-regulatory factors, IRF3 and IRF7 in Asian seabass, Lates calcarifer: Characterization, ontogeny and transcriptional modulation upon challenge with nervous necrosis virus
| Autor: | Vismai Naik T, Pani Prasad Kurcheti, Zahoor Mushtaq, K. Jeena, Rahul Krishnan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fish Proteins viruses Interferon Regulatory Factor-7 Aquatic Science Biology 03 medical and health sciences Fish Diseases Interferon Gene expression medicine Environmental Chemistry Animals Nodaviridae Amino Acid Sequence Gene Phylogeny Base Sequence Gene Expression Profiling virus diseases Promoter 04 agricultural and veterinary sciences General Medicine biochemical phenomena metabolism and nutrition Molecular biology DNA Virus Infections Immunity Innate Perciformes 030104 developmental biology Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries IRF7 Ectopic expression Interferon Regulatory Factor-3 IRF3 Sequence Alignment Interferon regulatory factors medicine.drug |
| Zdroj: | Fishshellfish immunology. 89 |
| ISSN: | 1095-9947 |
| Popis: | Interferon regulatory factor (IRF) 3 and IRF7 are key regulators of type I interferon (IFN) gene expression for the antiviral immune response. In the present study, interferon regulatory factor 3 and 7 from Asian seabass, namely AsIRF3 and AsIRF7 were cloned and characterized. The full-length cDNA sequence of IRF3 and IRF7 consisted of 2965 and 2343 bp respectively. AsIRF3 and AsIRF7 were true orthologes of vertebrate IRF3/7 and showed similar domain organization, with an N-terminal DBD which consisted five tryptophan residues in IRF3 and four in IRF7, a C-terminal IRF3 domain and a serine rich region. Both IRF3 and 7 constitutively expressed during the ontogenesis and in all tissues of healthy fish. The expression of both genes was up-regulated following NNV challenge with obvious transcript abundance in brain heart and kidney. Ectopic expression of AsIRF3 and AsIRF7 displayed activation of ISRE/NF-κB promoters and modulation of interferon, ISGs and pro-inflammatory cytokine gene expression. These observations indicated that IRF3 and IRF7 play an important role in Asian seabass's antiviral defense and the RIG-IRF-IFN axis is conserved in the species. |
| Databáze: | OpenAIRE |
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