Cadherin activity is required for activity-induced spine remodeling
| Autor: | Yasushi Hiraoka, Akihiko Taguchi, Naomasa Miki, Yoshiki Yagita, David R. Colman, Yoshinaga Saeki, Ling-Hui Zeng, Hidekazu Tanaka, Ko Okamura |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cytochalasin D
Dendritic spine Dendritic Spines Recombinant Fusion Proteins Green Fluorescent Proteins Synaptic Membranes Action Potentials Nerve Tissue Proteins AMPA receptor Neurotransmission Biology Hippocampus Synaptic Transmission Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Receptors AMPA Cells Cultured Research Articles 030304 developmental biology 0303 health sciences Microscopy Confocal Neuronal Plasticity Cadherin Excitatory Postsynaptic Potentials Cell Biology Cadherins Actins Rats Cell biology Apposition Microscopy Fluorescence chemistry Synaptic plasticity Excitatory postsynaptic potential 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200406030 |
| Popis: | Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone. |
| Databáze: | OpenAIRE |
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