Translation initiation factor IF2 of the myxobacterium Stigmatella aurantiaca: presence of a single species with an unusual N-terminal sequence
| Autor: | B Derijard, Yves Cenatiempo, Laure Bremaud, Soumaya Laalami |
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| Rok vydání: | 1997 |
| Předmět: |
Molecular Sequence Data
Mutant Gene Expression Sequence alignment Cross Reactions Prokaryotic Initiation Factor-2 medicine.disease_cause Microbiology Homology (biology) Species Specificity Peptide Initiation Factors medicine Amino Acid Sequence Myxococcales Cloning Molecular Stigmatella aurantiaca Molecular Biology Escherichia coli Peptide sequence Sequence Homology Amino Acid biology Prokaryotic initiation factor-2 Genetic Complementation Test Structural gene biology.organism_classification Molecular biology Biochemistry Genes Bacterial Sequence Alignment Research Article |
| Zdroj: | Journal of Bacteriology. 179:2348-2355 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | The structural gene for translation initiation factor IF2 (infB) was isolated from the myxobacterium Stigmatella aurantiaca on a 5.18-kb BamHI genomic restriction fragment. The infB gene (ca. 3.16 kb) encodes a 1,054-residue polypeptide with extensive homology within its G domain and C terminus with the equivalent regions of IF2s from Escherichia coli, Bacillus subtilis, Bacillus stearothermophilus, and Streptococcus faecium. The N-terminal region does not display any significant homology to other known proteins. The S. aurantiaca infB gene encodes a single protein which cross-reacted with antiserum to E. coli IF2 and was able to complement an E. coli infB mutant. The S. aurantiaca IF2 is distinguished from all other IF2s by a sequence of 160 residues near the N terminus that has an unusual composition, made up essentially of alanine, proline, valine, and glutamic acid. Within this sequence, the pattern PXXXAP is repeated nine times. Complete deletion of this sequence did not affect the factor's function in initiation of translation and even increased its capacity to complement the E. coli infB mutant. |
| Databáze: | OpenAIRE |
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