Bacteria localization and chorion thinning among preterm premature rupture of membranes

Autor: Amy P. Murtha, R. Phillips Heine, Liping Feng, Kimberly B. Fortner, Carla E. Ransom, Chad A. Grotegut, Rex C. Bentley, Lan Lan, Patrick C. Seed
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Fetal Membranes
Premature Rupture
Anatomy and Physiology
genetic structures
Gynecologic Infections
Colony Count
Microbial
lcsh:Medicine
Chorioamnionitis
Pathogenesis
Reproductive Physiology
Pregnancy
Microbial Physiology
Bacterial Physiology
lcsh:Science
In Situ Hybridization
Fluorescence
reproductive and urinary physiology
Multidisciplinary
Amnion
Microbial Growth and Development
Gestational age
Obstetrics and Gynecology
Chorion
Immunohistochemistry
Membrane
medicine.anatomical_structure
Infectious Diseases
embryonic structures
Medicine
Research Article
Adult
Gestational Age
Biology
Microbiology
Andrology
Fetal membrane
medicine
Humans
Least-Squares Analysis
Fetus
Bacteria
Preterm Labor
lcsh:R
Reproductive System
Bacteriology
medicine.disease
Pregnancy Complications
Immunology
lcsh:Q
Premature rupture of membranes
Zdroj: PLoS ONE, Vol 9, Iss 1, p e83338 (2014)
PLoS ONE
ISSN: 1932-6203
Popis: OBJECTIVE: Bacterial colonization of the fetal membranes and its role in pathogenesis of membrane rupture is poorly understood. Prior retrospective work revealed chorion layer thinning in preterm premature rupture of membranes (PPROM) subjects. Our objective was to prospectively examine fetal membrane chorion thinning and to correlate to bacterial presence in PPROM, preterm, and term subjects. STUDY DESIGN: Paired membrane samples (membrane rupture and membrane distant) were prospectively collected from: PPROM = 14, preterm labor (PTL = 8), preterm no labor (PTNL = 8), term labor (TL = 10), and term no labor (TNL = 8), subjects. Sections were probed with cytokeratin to identify fetal trophoblast layer of the chorion using immunohistochemistry. Fluorescence in situ hybridization was performed using broad range 16 s ribosomal RNA probe. Images were evaluated, chorion and choriodecidua were measured, and bacterial fluorescence scored. Chorion thinning and bacterial presence were compared among and between groups using Student's t-test, linear mixed effect model, and Poisson regression model (SAS Cary, NC). RESULTS: In all groups, the fetal chorion cellular layer was thinner at rupture compared to distant site (147.2 vs. 253.7 µm, p
Databáze: OpenAIRE
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