Postmortem diagnosis of cytomegalovirus and accompanying other infection agents by real-time PCR in cases of sudden unexpected death in infancy (SUDI)
| Autor: | Muzaffer Yildirim, Taner Daş, Ayse Ozgun, Nihan Ziyade, M. Feyzi Sahin, Sermet Koç, Gülhan Yağmur, Ferah Karayel, Neval Elgormus, Arzu Akçay |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology Palatine Tonsil Cytomegalovirus Salivary Glands 0302 clinical medicine Paraffin embedded tissues Prospective Studies Post-mortem microbiology 030212 general & internal medicine Forensic Pathology Lung Cause of death Brain virus diseases General Medicine Myocarditis Cytomegalovirus Infections Viruses Female Autopsy Meningitis Sudden Infant Death Encephalitis Forensic pathology medicine.medical_specialty Pneumonia Viral Congenital cytomegalovirus infection SUDI Real-Time Polymerase Chain Reaction Sialadenitis Article Pathology and Forensic Medicine 03 medical and health sciences 030225 pediatrics Pneumonia Bacterial medicine Humans Brain Chemistry Hepatitis business.industry Infant Newborn Infant medicine.disease DNA Viral Immunology business Law Real-time PCR |
| Zdroj: | Journal of Forensic and Legal Medicine |
| ISSN: | 1752-928X |
| DOI: | 10.1016/j.jflm.2015.11.008 |
| Popis: | As an opportunistic pathogen with high mortality rates, Cytomegalovirus (CMV) may lead to fatal disseminated CMV infection of the premature and newborn; thus necessitating the demonstration of CMV-DNA with clinical history and/or histopathological findings of CMV infection and defining other bacterial and viral infection agents with real-time polymerase chain reaction (RT-PCR) in udden unexpected death in infancy (SUDI) cases as we aimed in this study. 314 (144 female, 170 male) SUDI cases were prospectively investigated from January 2013 to January 2015 in Istanbul Forensic Medicine Institution. The study includes 87 tissue samples of 39 cases for post-mortem histopathological examination of interstitial pneumonia, myocarditis, meningitis, encephalitis, hepatitis, colitis or tubulointerstitial nephritis and/or accompanying chronic sialadenitis. CMV-DNA was found positive in 35 (40.2%) salivary gland, 19 (21.8%) lung, 1 (1.1%) tonsil, and 1 (1.1%) brain tissues. CMV sialadenitis and/or CMV pneumonia associated with other viral and/or bacterial agents were detected in 23 (60%) of 39 infant cases. The demonstration of CMV-DNA would significantly clarify the cause of death and collection of epidemiological data in SUDI cases with clinical history and histopathological findings of CMV infection accompanying chronic CMV sialadenitis. Furthermore, CMV suppresses the immune system, and may predispose to other bacterial and/or viral infections in these cases. Post-mortem molecular investigations are useful in explaining cause of death in SUDI with a suspicion of infection in forensic autopsies. Highlights • CMV may lead to fatal disseminated CMV infection of the premature and newborn. • Our study is the first comprehensive study investigating CMV infections in SUDI. • CMV suppresses the immune system, and may predispose to other infections. • Postmortem molecular investigations are useful in explaining cause of death in SUDI. |
| Databáze: | OpenAIRE |
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