Effective number of breeders from sibship reconstruction: empirical evaluations using hatchery steelhead
Autor: | Brian K. Hand, Matthew R. Campbell, Jesse McCane, Gordon Luikart, Robin S. Waples, Craig A. Steele, Michael W. Ackerman, Ryan K. Waples, Brittany A. Garner |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Conservation genetics sibship assignment PwoP Pedigree chart Biology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Effective population size Statistics Genetic model Genetics Ecology Evolution Behavior and Systematics genetic monitoring Estimator Original Articles sibship reconstruction COLONY Mating system 030104 developmental biology conservation genetics Sample size determination Oncorhynchus mykiss Original Article General Agricultural and Biological Sciences effective population size Genetic monitoring |
Zdroj: | Evolutionary Applications |
ISSN: | 1752-4571 |
DOI: | 10.1111/eva.12433 |
Popis: | Effective population size (Ne) is among the most important metrics in evolutionary biology. In natural populations, it is often difficult to collect adequate demographic data to calculate Ne directly. Consequently, genetic methods to estimate Ne have been developed. Two Ne estimators based on sibship reconstruction using multi-locus genotype data have been developed in recent years; sibship assignment and parentage analysis without parents. In this study, we evaluated the accuracy of sibship reconstruction using a large empirical dataset from five hatchery steelhead populations with known pedigrees and using 95 single nucleotide polymorphism (SNP) markers. We challenged the software COLONY with 2,599,961 known relationships and demonstrated that reconstruction of full-sib and unrelated pairs were greater than 95% and 99% accurate, respectively. However, reconstruction of half-sib pairs was poor (< 5% accurate). Despite poor half-sib reconstruction, both estimators provided accurate estimates of the effective number of breeders (Nb) when sample sizes were near or greater than the true Nb and when assuming a monogamous mating system. We further demonstrated that both methods provide roughly equivalent estimates of Nb. Our results indicate that sibship reconstruction and current SNP panels provide promise for estimating Nb in steelhead populations in the region. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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