Selective killing of smooth muscle cells in culture by the ricin A-chain conjugated with monoclonal antibodies to a cell surface antigen via a dextran bridge
| Autor: | V. P. Torchilin, A. V. Maksimenko, A. I. Faerman, O. Yu. Printseva, O. B. Ilynsky, A. G. Tonevitsky |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.drug_class
Cell Survival Ricin Biology Monoclonal antibody Muscle Smooth Vascular Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Antigen In vivo Leucine medicine Animals Molecular Biology Aorta Pharmacology Mice Inbred BALB C Antibodies Monoclonal Dextrans Cell Biology Molecular biology In vitro Dextran Biochemistry chemistry Cell culture Antigens Surface Chromatography Gel Molecular Medicine Conjugate |
| Zdroj: | Experientia. 41(10) |
| ISSN: | 0014-4754 |
| Popis: | Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction of cultured cells with the conjugates obtained and with control substances was monitored following incorporation of 14C-leucine radioactivity. It was found that 14C-leucine incorporation was suppressed by 80-90% at a conjugate concentration of 10(-6)-10(-7) M. Antigen-negative cells (line IAR; rat hepatocytes) were insensitive to the conjugate at any concentration used. Control use of purified ricin A-chain, native or oxidized dextran, specific and nonspecific IgG did not affect normal 14C-leucine incorporation. The data obtained may be useful for designing targeted drug transport systems and for selective screening of modulated smooth cells in vascular pathology models in vivo. |
| Databáze: | OpenAIRE |
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