Interleukin-12 p40 Gene Expression Is Induced in Lipopolysaccharide-Activated Pituitary Glands in vivo

Autor: Kun-yong Kim, Inpyo Choi, Hyun Kim, Sun Mi Shin, Kwang Ho Pyun, Seung Hyun Han, Changmee Kim, Young Yang
Rok vydání: 2002
Předmět:
Lipopolysaccharides
Male
endocrine system
medicine.medical_specialty
Lipopolysaccharide
Endocrinology
Diabetes and Metabolism
Lipopolysaccharide Receptors
Gene Expression
macromolecular substances
Proinflammatory cytokine
Rats
Sprague-Dawley
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Organ Culture Techniques
Endocrinology
Immune system
Pituitary Gland
Anterior
In vivo
Internal medicine
Receptors
Adrenergic
beta
Gene expression
medicine
Animals
RNA
Messenger
In Situ Hybridization
Regulation of gene expression
Interleukin-12 Subunit p40
Reverse Transcriptase Polymerase Chain Reaction
Endocrine and Autonomic Systems
Chemistry
NF-kappa B
Interleukin
DNA
Blotting
Northern
Interleukin-12
Rats
Transcription Factor AP-1
Protein Subunits
nervous system
Pituitary Gland
Interleukin 12
hormones
hormone substitutes
and hormone antagonists
Zdroj: Neuroendocrinology. 75:347-357
ISSN: 1423-0194
0028-3835
DOI: 10.1159/000059431
Popis: Proinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-ĸB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
Databáze: OpenAIRE
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