Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing
| Autor: | A Kawai, Takashi Nishida, Koji Asaumi, Masaharu Takigawa, M Asano, Hajime Inoue, Tomoichiro Yamaai, S Mitani, E Nakata, Tohru Nakanishi |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Connective tissue Bone healing Biology Chondrocyte Immediate-Early Proteins Mice medicine Animals RNA Messenger Endochondral ossification In Situ Hybridization Fracture Healing Mice Inbred ICR Base Sequence integumentary system Cartilage Growth factor Connective Tissue Growth Factor Blotting Northern Immunohistochemistry Molecular biology CTGF medicine.anatomical_structure Intramembranous ossification Intercellular Signaling Peptides and Proteins DNA Probes |
| Zdroj: | Bone. 31:441-447 |
| ISSN: | 8756-3282 |
| Popis: | Localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing in mouse ribs were investigated. In situ hybridization demonstrated that CTGF/Hcs24 mRNA was remarkably expressed, especially in hypertrophic chondrocytes and proliferating chondrocytes, in the regions of regenerating cartilage on days 8 and 14 after fracture. CTGF/Hcs24 mRNA was also expressed in proliferating periosteal cells in the vicinity of the fracture sites on days 2 and 8, and in cells in fibrous tissue around the callus on day 8. Northern blot analysis showed that expression of CTGF/Hcs24 mRNA was 3.9 times higher on day 2 of fracture healing than that on day 0. On day 8, it reached a peak of 8.6 times higher than that on day 0. It then declined to a lower level. Immunostaining showed that CTGF/Hcs24 was localized in hypertrophic chondrocytes and proliferating chondrocytes in the regions of regenerating cartilage, and in active osteoblasts in the regions of intramembranous ossification. Although CTGF/Hcs24 was abundant in the proliferating and differentiating cells (on days 8 and 14), immunostaining decreased as the cells differentiated to form bone (on day 20). CTGF/Hcs24 was also detected in cells in fibrous tissue, vascular endothelial cells in the callus, and periosteal cells around the fracture sites. These results suggest that CTGF/Hcs24 plays some role in fracture healing. |
| Databáze: | OpenAIRE |
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