An Increase in Membrane Cholesterol by Graphene Oxide Disrupts Calcium Homeostasis in Primary Astrocytes

Autor: Fabrizia Cesca, Ester Vázquez, Cristina Martin Jimenez, Deepali D. Kale, Stefano Ferroni, Tiziano Bandiera, Fabio Benfenati, Andrea Armirotti, Mattia Bramini, Anna Rocchi, Martina Chiacchiaretta
Přispěvatelé: Bramini M., Chiacchiaretta M., Armirotti A., Rocchi A., Kale D.D., Martin C., Vazquez E., Bandiera T., Ferroni S., Cesca F., Benfenati F., Bramini, Mattia, Chiacchiaretta, Martina, Armirotti, Andrea, Rocchi, Anna, Kale, Deepali D, Martin, Cristina, Vázquez, Ester, Bandiera, Tiziano, Ferroni, Stefano, Cesca, Fabrizia, Benfenati, Fabio
Rok vydání: 2019
Předmět:
Proteome
Intracellular Space
02 engineering and technology
01 natural sciences
law.invention
Rats
Sprague-Dawley
chemistry.chemical_compound
law
Homeostasis
General Materials Science
Cells
Cultured
Chemistry
Cell Cycle
021001 nanoscience & nanotechnology
3. Good health
Cell biology
medicine.anatomical_structure
Biological Physics (physics.bio-ph)
Neurons and Cognition (q-bio.NC)
Graphite
0210 nano-technology
Intracellular
Biotechnology
Astrocyte
astrocytes
calcium
cholesterol
graphene
proteomics
Cell Survival
Central nervous system
Oxide
FOS: Physical sciences
010402 general chemistry
Biomaterials
astrocytes
calcium
cholesterol
graphene
proteomics
astrocyte
medicine
Animals
Physics - Biological Physics
Viability assay
Calcium Signaling
Cell Proliferation
Calcium metabolism
Graphene
Cell Membrane
General Chemistry
0104 chemical sciences
Quantitative Biology - Neurons and Cognition
FOS: Biological sciences
Astrocytes
Lipidomics
Zdroj: Small
ISSN: 1613-6810
DOI: 10.1002/smll.201900147
Popis: The use of graphene nanomaterials (GNMs) for biomedical applications targeted to the central nervous system is exponentially increasing, although precise information on their effects on brain cells is lacking. In this work, we addressed the molecular changes induced in cortical astrocytes by few-layer graphene (FLG) and graphene oxide (GO) flakes. Our results show that exposure to FLG/GO does not affect cell viability or proliferation. However, proteomic and lipidomic analyses unveiled alterations in several cellular processes, including intracellular Ca2+ ([Ca2+]i) homeostasis and cholesterol metabolism, which were particularly intense in cells exposed to GO. Indeed, GO exposure impaired spontaneous and evoked astrocyte [Ca2+]i signals and induced a marked increase in membrane cholesterol levels. Importantly, cholesterol depletion fully rescued [Ca2+]i dynamics in GO-treated cells, indicating a causal relationship between these GO-mediated effects. Our results indicate that exposure to GNMs alters intracellular signaling in astrocytes and may impact on astrocyte-neuron interactions.
Comment: This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in Small after peer review. To access the final edited and published work see https://onlinelibrary.wiley.com/doi/10.1002/smll.201900147 40 pages, 6 main figures and 1 supplementary figure
Databáze: OpenAIRE
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