An Increase in Membrane Cholesterol by Graphene Oxide Disrupts Calcium Homeostasis in Primary Astrocytes
Autor: | Fabrizia Cesca, Ester Vázquez, Cristina Martin Jimenez, Deepali D. Kale, Stefano Ferroni, Tiziano Bandiera, Fabio Benfenati, Andrea Armirotti, Mattia Bramini, Anna Rocchi, Martina Chiacchiaretta |
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Přispěvatelé: | Bramini M., Chiacchiaretta M., Armirotti A., Rocchi A., Kale D.D., Martin C., Vazquez E., Bandiera T., Ferroni S., Cesca F., Benfenati F., Bramini, Mattia, Chiacchiaretta, Martina, Armirotti, Andrea, Rocchi, Anna, Kale, Deepali D, Martin, Cristina, Vázquez, Ester, Bandiera, Tiziano, Ferroni, Stefano, Cesca, Fabrizia, Benfenati, Fabio |
Rok vydání: | 2019 |
Předmět: |
Proteome
Intracellular Space 02 engineering and technology 01 natural sciences law.invention Rats Sprague-Dawley chemistry.chemical_compound law Homeostasis General Materials Science Cells Cultured Chemistry Cell Cycle 021001 nanoscience & nanotechnology 3. Good health Cell biology medicine.anatomical_structure Biological Physics (physics.bio-ph) Neurons and Cognition (q-bio.NC) Graphite 0210 nano-technology Intracellular Biotechnology Astrocyte astrocytes calcium cholesterol graphene proteomics Cell Survival Central nervous system Oxide FOS: Physical sciences 010402 general chemistry Biomaterials astrocytes calcium cholesterol graphene proteomics astrocyte medicine Animals Physics - Biological Physics Viability assay Calcium Signaling Cell Proliferation Calcium metabolism Graphene Cell Membrane General Chemistry 0104 chemical sciences Quantitative Biology - Neurons and Cognition FOS: Biological sciences Astrocytes Lipidomics |
Zdroj: | Small |
ISSN: | 1613-6810 |
DOI: | 10.1002/smll.201900147 |
Popis: | The use of graphene nanomaterials (GNMs) for biomedical applications targeted to the central nervous system is exponentially increasing, although precise information on their effects on brain cells is lacking. In this work, we addressed the molecular changes induced in cortical astrocytes by few-layer graphene (FLG) and graphene oxide (GO) flakes. Our results show that exposure to FLG/GO does not affect cell viability or proliferation. However, proteomic and lipidomic analyses unveiled alterations in several cellular processes, including intracellular Ca2+ ([Ca2+]i) homeostasis and cholesterol metabolism, which were particularly intense in cells exposed to GO. Indeed, GO exposure impaired spontaneous and evoked astrocyte [Ca2+]i signals and induced a marked increase in membrane cholesterol levels. Importantly, cholesterol depletion fully rescued [Ca2+]i dynamics in GO-treated cells, indicating a causal relationship between these GO-mediated effects. Our results indicate that exposure to GNMs alters intracellular signaling in astrocytes and may impact on astrocyte-neuron interactions. Comment: This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in Small after peer review. To access the final edited and published work see https://onlinelibrary.wiley.com/doi/10.1002/smll.201900147 40 pages, 6 main figures and 1 supplementary figure |
Databáze: | OpenAIRE |
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