Validation of an immunoperoxidase monolayer assay for total anti–Vaccinia virus antibody titration
| Autor: | Maria Isabel Maldonado Coelho Guedes, Ana Carolina Diniz Matos, Priscilla F. Gerber, Marieta C. Madureira, Marcos Xavier Silva, Zélia Inês Portela Lobato |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
viruses
Cattle Diseases Vaccinia virus Biology Antibodies Viral Sensitivity and Specificity Virus Immunoenzyme Techniques chemistry.chemical_compound Plaque reduction neutralization test Neutralization Tests Vaccinia Animals Orthopoxvirus Bovine serum albumin General Veterinary Immunoperoxidase Reproducibility of Results virus diseases biology.organism_classification Virology Vaccinia virus antibody chemistry biology.protein Cattle Female Antibody Brazil |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 24:355-358 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/1040638711435231 |
| Popis: | Vaccinia virus (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were −1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|