Analysis of myeloperoxidase promotor polymorphism and enzyme activity in Turkish patients with vitiligo
| Autor: | A. Binnur Erbagci, E Ilker Saygili, Ş. Nur Aksoy, Zülal Erbagci, Sacide Pehlivan, Tugce Sever |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Genetic Markers Male Adolescent Free Radicals Turkey Vitiligo Enzyme-Linked Immunosorbent Assay Dermatology medicine.disease_cause Sensitivity and Specificity Severity of Illness Index Pathogenesis Genotype medicine Humans Child skin and connective tissue diseases Pigmentation disorder Peroxidase Polymorphism Genetic integumentary system biology Reverse Transcriptase Polymerase Chain Reaction business.industry Middle Aged medicine.disease Enzyme assay Hypochlorous Acid Oxidative Stress Case-Control Studies Myeloperoxidase Immunology biology.protein Female Gene polymorphism business Oxidative stress |
| Zdroj: | European Journal of Dermatology. 19:576-580 |
| ISSN: | 1952-4013 1167-1122 |
| DOI: | 10.1684/ejd.2009.0793 |
| Popis: | Vitiligo is an acquired depigmentary disorder characterized by white areas of the skin due to loss of epidermal melanocytes. Oxidative stress and free radicals are suggested as important phenomena in the pathogenesis of vitiligo. Myeloperoxidase is a lysosomal enzyme of polymorphonuclear leukocytes and acts as a catalyst in the production of hypochlorous acid, a powerful oxidant. In this study we analysed enzyme activity and gene polymorphism of myeloperoxidase in patients with vitiligo. Fifty-four patients with vitiligo and 58 healthy controls were enrolled to this study. Patient groups were subdivided according to localization of the lesions; generalized, acrofacial and local. Plasma myeloperoxidase enzyme activity was determined with ELISA and G-463A gene (-463) polymorphism with the PCR-RFLP (AciI) method. The plasma MPO level was significantly lower in vitiligo patients than in the healthy controls (p = 0.005), however, it was not significantly different among subtypes of vitiligo (p = 0.8). A significant difference was not observed for G-463A genotype and allele distribution in patients with vitiligo. In conclusion, the present study is the first study investigating MPO G-463A polymorphism and enzyme levels, which warrants further studies with higher patient numbers and broader polymorphism panels. |
| Databáze: | OpenAIRE |
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