Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes
| Autor: | Katarzyna Michalak-Micka, Martin Meuli, Thomas Biedermann, Sophie Böttcher-Haberzeth, Agnes S. Klar, Ernst Reichmann |
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| Přispěvatelé: | University of Zurich, Biedermann, Thomas |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Keratinocytes Male Pathology medicine.medical_specialty Stromal cell Adolescent Adipose tissue 610 Medicine & health Young Adult 03 medical and health sciences Foreskin 0302 clinical medicine 030225 pediatrics medicine Humans 10220 Clinic for Surgery 2735 Pediatrics Perinatology and Child Health Child Fibroblast Cells Cultured Aged Skin Artificial Tissue Engineering integumentary system Epidermis (botany) business.industry Mesenchymal stem cell Infant Cell Differentiation Dermis Skin Transplantation General Medicine Fibroblasts Middle Aged 2746 Surgery medicine.anatomical_structure Child Preschool Pediatrics Perinatology and Child Health Female 030211 gastroenterology & hepatology Surgery Epidermis Stromal Cells Keratinocyte business Transglutaminase 5 |
| Zdroj: | Pediatric Surgery International. 35:121-127 |
| ISSN: | 1437-9813 0179-0358 |
| DOI: | 10.1007/s00383-018-4383-5 |
| Popis: | The use of autologous bio-engineered dermo-epidermal skin substitutes (DESS) yields a pivotal opportunity to cover large skin defects in human patients. These skin grafts consist of both epidermal and dermal compartments necessary for robust and permanent functional wound closure. In this study, we investigated the impact of mesenchymal cells derived from different body site origins on the expression pattern of diverse markers within DESS. Human keratinocytes were obtained from interfollicular epidermis, and mesenchymal cells were isolated from foreskin, palmar skin, fat tissue, and tonsils. After expansion, epidermal cells were seeded on collagen I hydrogels containing stromal cells. These human DESS were transplanted on the back of immune-incompetent rats. After 3 weeks, transplants were excised and analyzed using immunohistology techniques. The macroscopic appearance of skin grafts containing tonsil, fat tissue, or palmar derived mesenchymal cells, was similar to substitutes with foreskin derived dermal fibroblasts. All skin grafts had a strong membrane-localized expression of Lingo-1 in the epidermis. Additionally, we observed an intense expression of transglutaminase 5 in upper epidermal cell layers of the skin grafts confirming a proper keratinocyte differentiation. Tropoelastin was localized throughout the dermal compartments and tightly in contact with the dermo-epidermal junction suggesting an advanced maturation of all skin grafts. Our data implicate that stromal cells derived from tonsil, fat tissue, and palmar skin can assume fibroblast functions supporting keratinocyte proliferation and differentiation. These findings indicate that distinct types of mesenchymal cells can be clinically used for skin engineering purposes. |
| Databáze: | OpenAIRE |
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