Formation of cellular projections in neural progenitor cells depends on SK3 channel activity
| Autor: | Stephan Grissmer, Christian Proepper, Stefan Liebau, Tobias M. Boeckers, Alexander Storch, Bianca Vaida, Paul Dietl, Oliver H. Wittekindt |
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| Rok vydání: | 2007 |
| Předmět: |
Patch-Clamp Techniques
Small-Conductance Calcium-Activated Potassium Channels Scorpion Venoms Biology Biochemistry Membrane Potentials Rats Sprague-Dawley Potassium Channels Calcium-Activated Cellular and Molecular Neuroscience Mesencephalon Pregnancy Scyllatoxin Animals Drug Interactions Pseudopodia RNA Messenger Progenitor cell Microscopy Immunoelectron Cells Cultured Ion channel Neurons Reverse Transcriptase Polymerase Chain Reaction Stem Cells Embryo Mammalian Immunohistochemistry Neural stem cell Calcium-activated potassium channel Rats Dendritic filopodia Cell biology Calcium Channel Agonists stomatognathic diseases Benzimidazoles Female Lamellipodium Neuroscience Filopodia |
| Zdroj: | Journal of Neurochemistry. 101:1338-1350 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.2006.04437.x |
| Popis: | Ion channels are potent modulators for developmental processes in progenitor cells. In a screening approach for different ion channels in neural progenitor cells (NPCs) we observed a 1-ethyl-2-benzimidazolinone (1-EBIO) activated inward current, which could be blocked by scyllatoxin (ScTX, IC50 = 2 ± 0.3 nmol/L). This initial evidence for the expression of the small conductance Ca2+ activated K+-channel SK3 was confirmed by the detection of SK3 transcripts and protein in NPCs. Interestingly, SK3 proteins were highly expressed in non-differentiated NPCs with a focused localization in lamellipodia as well as filopodial structures. The activation of SK3 channels using 1-EBIO lead to an immediate filopodial sprouting and the translocation of the protein into these novel filopodial protrusions. Both effects could be prevented by the pre-incubation of NPCs with ScTX. Our study gives first evidence that the formation and prolongation of filopodia in NPCs is, at least in part, effectively induced and regulated by SK3 channels. |
| Databáze: | OpenAIRE |
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