Tax-mediated re-routing of the HTLV-1 p13 protein to nuclear speckles
| Autor: | Genoveffa Franchini, Cynthia A. Pise-Masison, Robyn Washington Parks, Vibeke Andresen, Uma Sinha-Datta, Risaku Fukumoto, Valentina Cecchinato, Christophe Nicot |
|---|---|
| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Retrovirology Retrovirology, Vol 8, Iss Suppl 1, p A125 (2011) |
| ISSN: | 1742-4690 |
| DOI: | 10.1186/1742-4690-8-s1-a125 |
| Popis: | Human T-cell Leukemia Virus type-1 (HTLV-1) is highly immunogenic and has low variability so tight regulation of its expression is essential for virus maintenance in vivo. HTLV-1 replication is positively regulated by Tax and Rex, and negatively by the p30 and HBZ proteins. Here, we demonstrate that HTLV-1 encodes another negative regulator of virus expression, the p13 protein. Expressed separately, p13 localizes to the mitochondria, but in the presence of Tax, p13 is stabilized, and re-routed to the nuclear speckles. The p13 protein directly binds Tax, decreases Tax binding to the CBP/ p300 transcriptional co-activator and, by reducing Tax transcriptional activity, suppresses viral expression. These findings suggest that HTLV-1 has evolved a complex mechanism to control its own replication through regulation of positive and negative viral proteins. Further, these results emphasize the importance of studying the function of the HTLV-1 viral proteins, not only in isolation, but also in the context of full viral replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|