Oxidative DNA damage in chronic renal failure patients
| Autor: | Naouale El-Yamani, Elisabet Coll, José Ballarín, Noel Xamena, Joselyn Reyes, Liliana Zúñiga, Ricard Marcos, Susana Pastor, Silvia Berenice Sandoval, Enrique Andrés, Elitsa Stoyanova |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Genome instability medicine.medical_specialty Time Factors DNA damage medicine.medical_treatment Renal function oxidative damage chemistry.chemical_compound comet assay Renal Dialysis Internal medicine medicine Humans haemodialysis treatment Dialysis Aged Transplantation Creatinine uraemic state business.industry CRF DNA Middle Aged medicine.disease Comet assay Oxidative Stress Cross-Sectional Studies Endocrinology chemistry Nephrology Kidney Failure Chronic Female Comet Assay Hemodialysis business Oxidation-Reduction DNA Damage Kidney disease |
| Zdroj: | NEPHROLOGY DIALYSIS TRANSPLANTATION r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| ISSN: | 1460-2385 0931-0509 |
| DOI: | 10.1093/ndt/gfp575 |
| Popis: | Background. Chronic renal failure (CRF) patients present a high incidence of cardiovascular pathologies and cancer. This has been attributed to the existence of genomic instability in these patients, and consequently they should present elevated levels of genetic damage. Methods. To determine the background levels of genetic damage and its specific levels of oxidative damage, a large population of 253 CRF patients (77 in dialysis) was analysed using the comet assay. The percentage of DNA in the tail was used as a measure of basal genetic damage. In addition, the use of endo III and FPG enzymes allowed us to determine the levels of specific oxidative damage in DNA bases. Results. This is the first study that uses endo III and FPG enzymes to measure oxidative damage in CRF patients. Overall genetic damage, as well as specific oxidative damage, was higher in dialysis patients than in the CRF patients with different stages of uraemic state; genetic damage increased when serum creatinine levels increased. Genomic damage in dialysis patients decreased in those patients submitted to dialysis for a long time. Conclusions. Genetic damage increases when renal function decreases, being maximum in haemodialysis patients. Although part of the observed damage can be attributed to the uraemic state itself, other individual genetic factors can influence a state of genomic instability responsible for the observed genomic damage. |
| Databáze: | OpenAIRE |
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