Evidence of an Intracellular Reservoir in the Nasal Mucosa of Patients with RecurrentStaphylococcus aureusRhinosinusitis

Autor: Sophie Clément, Patrice Francois, Daniel Pablo Lew, Elzbieta Huggler, Pierre Vaudaux, Christine Chaponnier, Jean-Sylvain Lacroix, Jacques Schrenzel, Sandy Kampf
Rok vydání: 2005
Předmět:
Staphylococcal Infections/ microbiology/pathology
Staphylococcus aureus
medicine.drug_class
Biopsy
Epithelium/microbiology
Antibiotics
Mucous membrane of nose
medicine.disease_cause
Epithelium
Nasal Mucosa/ microbiology/pathology
03 medical and health sciences
Recurrence
medicine
Humans
Immunology and Allergy
Sinusitis
Nose
Rhinitis
030304 developmental biology
ddc:616
Fibroblasts/microbiology
First episode
0303 health sciences
Microscopy
Confocal
030306 microbiology
business.industry
Intracellular parasite
Staphylococcus aureus/ isolation & purification
Fibroblasts
Staphylococcal Infections
medicine.disease
Rhinitis/ microbiology/pathology
Sinusitis/ microbiology/pathology
3. Good health
Microscopy
Electron
Nasal Mucosa
Infectious Diseases
Paranasal sinuses
medicine.anatomical_structure
Carrier State
Immunology
business
Zdroj: The Journal of Infectious Diseases, Vol. 192, No 6 (2005) pp. 1023-1028
ISSN: 1537-6613
0022-1899
DOI: 10.1086/432735
Popis: Severe infections due to Staphylococcus aureus require prolonged therapy for cure, and relapse may occur even years after the first episode. Persistence of S. aureus may be explained, in part, by nasal carriage of S. aureus, which occurs in a large percentage of healthy humans and represents a major source of systemic infection. However, the persistence of internalized S. aureus within mucosal cells has not been evaluated in humans. Here, we provide the first in vivo evidence of intracellular reservoirs of S. aureus in humans, which were assessed in endonasal mucosa specimens from patients suffering from recurrent S. aureus rhinosinusitis due to unique, patient-specific bacterial clonotypes. Heavily infected foci of intracellular bacteria located in nasal epithelium, glandular, and myofibroblastic cells were revealed by inverted confocal laser scan fluorescence and electron microscopic examination of posttherapy intranasal biopsy specimens from symptom-free patients undergoing surgery on the sinuses. Intracellular residence may provide a sanctuary for pathogenic bacteria by protecting them from host defense mechanisms and antibiotic treatment during acute, recurrent S. aureus rhinosinusitis.
Databáze: OpenAIRE
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