Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales
| Autor: | Wileman, TM, Weinert, LA, Howell, KJ, Wang, J, Peters, SE, Williamson, SM, Wells, JM, Langford, PR, Rycroft, AN, Wren, BW, Maskell, DJ, Tucker, AW, Bosse, JT, Li, Y, Cuccui, J, Terra, VS, Beddow, J, Maglennon, GA, Chaudhuri, RR |
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| Přispěvatelé: | Howell, Kate J [0000-0001-8069-920X], Apollo - University of Cambridge Repository, Biotechnology and Biological Sciences Research Council |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
pathotyping
Streptococcus suis Swine Palatine Tonsil Disease Virulence markers BACTERIAL PATHOGENS Clinical Veterinary Microbiology PIGS 11 Medical and Health Sciences Swine Diseases 0303 health sciences Surveillance Virulence virulence markers England Molecular Diagnostic Techniques Carrier State surveillance LATEST DEVELOPMENTS Life Sciences & Biomedicine Microbiology (medical) Genetic Markers Biology Microbiology molecular diagnostics 03 medical and health sciences 07 Agricultural and Veterinary Sciences Streptococcal Infections Multiplex polymerase chain reaction Molecular diagnostics THIOL-ACTIVATED HEMOLYSIN Animals Host-Microbe Interactomics 030304 developmental biology VLAG Science & Technology Wales IDENTIFICATION 030306 microbiology CAPSULAR TYPES STRAINS 06 Biological Sciences biology.organism_classification Carriage Genetic marker WIAS EXTRACELLULAR PROTEIN Pathotyping Asymptomatic carrier MURAMIDASE-RELEASED PROTEIN Multiplex Polymerase Chain Reaction Genome Bacterial |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, 57(7) Journal of Clinical Microbiology 57 (2019) 7 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Streptococcus suis is one of the most important zoonotic bacterial pathogens of pigs, causing significant economic losses to the global swine industry. S. suis is also a very successful colonizer of mucosal surfaces, and commensal strains can be found in almost all pig populations worldwide, making detection of the S. suis species in asymptomatic carrier herds of little practical value in predicting the likelihood of future clinical relevance. Streptococcus suis is one of the most important zoonotic bacterial pathogens of pigs, causing significant economic losses to the global swine industry. S. suis is also a very successful colonizer of mucosal surfaces, and commensal strains can be found in almost all pig populations worldwide, making detection of the S. suis species in asymptomatic carrier herds of little practical value in predicting the likelihood of future clinical relevance. The value of future molecular tools for surveillance and preventative health management lies in the detection of strains that genetically have increased potential to cause disease in presently healthy animals. Here we describe the use of genome-wide association studies to identify genetic markers associated with the observed clinical phenotypes (i) invasive disease and (ii) asymptomatic carriage on the palatine tonsils of pigs on UK farms. Subsequently, we designed a multiplex PCR to target three genetic markers that differentiated 115 S. suis isolates into disease-associated and non-disease-associated groups, that performed with a sensitivity of 0.91, a specificity of 0.79, a negative predictive value of 0.91, and a positive predictive value of 0.79 in comparison to observed clinical phenotypes. We describe evaluation of our pathotyping tool, using an out-of-sample collection of 50 previously uncharacterized S. suis isolates, in comparison to existing methods used to characterize and subtype S. suis isolates. In doing so, we show our pathotyping approach to be a competitive method to characterize S. suis isolates recovered from pigs on UK farms and one that can easily be updated to incorporate global strain collections. |
| Databáze: | OpenAIRE |
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