Synemin Isoforms in Astroglial and Neuronal Cells from Human Central Nervous System
| Autor: | A, Izmiryan, E, Peltekian, T, Federici, D, Paulin, Z L, Li, Z G, Xue |
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| Rok vydání: | 2009 |
| Předmět: |
Nervous system
Vimentin macromolecular substances Biochemistry Cellular and Molecular Neuroscience Intermediate Filament Proteins medicine Animals Humans Protein Isoforms Intermediate filament Cells Cultured Neurons biology Chemistry Synemin Brain Neural crest General Medicine Nestin Immunohistochemistry Rats Cell biology medicine.anatomical_structure nervous system Astrocytes biology.protein NeuN Astrocyte |
| Zdroj: | Neurochemical Research. 35:881-887 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-009-0111-9 |
| Popis: | The intermediate filament (IF) synemin gene encodes three IF proteins (H 180, M 150, L 41 kDa) with overlapping distributions. Synemin M was present early with vimentin and nestin. Synemin H was found later in the nervous system and mesodermic derivatives concomitantly with angiogenesis and the migration of neural crest cells. Synemin L appeared later in neurons. A series of in vitro cell cultures were done to identify the linkage between synemin isoforms and specific cell types of the central nervous system (CNS). The neurons and glia from the brains of humans and rats were cultured and double immunostaining done with antibodies against the H/M or L synemin isoforms and neural cell types (betaIII-tubulin or NeuN) or astrocyte intermediate filaments (GFAP or vimentin). In neurons of the CNS, synemin H/M were co-expressed with GFAP, vimentin or nestin in glial cells, whereas synemin L was found in neurons. |
| Databáze: | OpenAIRE |
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