Recombinant RquA catalyzes the in vivo conversion of ubiquinone to rhodoquinone in Escherichia coli and Saccharomyces cerevisiae
| Autor: | Evan J. Jacobs, Catherine F. Clarke, Ann C. Bernert, Christina C.Y. Choi, Michelle C. Bradley, Jennifer N. Shepherd, Paloma M. Roberts Buceta, Samantha R. Reinl, Carly R. Goodspeed, John C. Culver, Gilles J. Basset |
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| Rok vydání: | 2019 |
| Předmět: |
Anaerobic respiration
Ubiquinone Saccharomyces cerevisiae Respiratory chain medicine.disease_cause Biosynthesis Rhodospirillum rubrum Medical and Health Sciences Article Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins medicine Genetics Escherichia coli Molecular Biology Gene 030304 developmental biology Fumarate reduction 0303 health sciences biology 030306 microbiology Chemistry Cell Biology Biological Sciences biology.organism_classification Reverse genetics Recombinant Proteins Biosynthetic Pathways Biochemistry Rhodoquinone Oxidation-Reduction |
| Zdroj: | Biochimica et biophysica acta. Molecular and cell biology of lipids, vol 1864, iss 9 |
| ISSN: | 1879-2618 |
| Popis: | Terpenoid quinones are liposoluble redox-active compounds that serve as essential electron carriers and anti-oxidants. One such quinone, rhodoquinone (RQ), couples the respiratory electron transfer chain to the reduction of fumarate to facilitate anaerobic respiration. This mechanism allows RQ-synthesizing organisms to operate their respiratory chain using fumarate as a final electron acceptor. RQ biosynthesis is restricted to a handful of prokaryotic and eukaryotic organisms, and details of this biosynthetic pathway remain enigmatic. One gene, rquA, was discovered to be required for RQ biosynthesis in Rhodospirillum rubrum. However, the function of the gene product, RquA, has remained unclear. Here, using reverse genetics approaches, we demonstrate that RquA converts ubiquinone to RQ directly. We also demonstrate the first in vivo synthetic production of RQ in Escherichia coli and Saccharomyces cerevisiae, two organisms that do not natively produce RQ. These findings help clarify the complete RQ biosynthetic pathway in species which contain RquA homologs. |
| Databáze: | OpenAIRE |
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