Organoids from pituitary as a novel research model toward pituitary stem cell exploration

Autor: Benjamin Beck, Hugo Vankelecom, Hiroshi Uji-i, Matteo Boretto, Annelies Vennekens, Emma Laporte, Alizée Vercauteren Drubbel, Indra Van Zundert, Benoit Cox, Heleen Roose, Charlotte Nys, Hiroto Kobayashi
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
WNT pathway
Endocrinology
Diabetes and Metabolism
Cell Culture Techniques
Gene Expression
Mice
SCID
Pituitary Gland -- cytology
Stem cell marker
MOUSE
pituitary
Stem Cells -- cytology -- metabolism
0302 clinical medicine
Endocrinology
Mice
Inbred NOD
SPECIFICATION
Cells
Cultured
organoids
Stem Cells
Wnt signaling pathway
Cell Differentiation
Sciences bio-médicales et agricoles
Phenotype
Cell biology
Organoids
Pituitary Gland
Stem cell
CONTRIBUTE
Life Sciences & Biomedicine
Endocrine gland
EXPRESSION
medicine.medical_specialty
EPITHELIUM
Mice
Transgenic
Biology
Stem Cell Transplantation -- methods
STEM/PROGENITOR CELL
03 medical and health sciences
Endocrinology & Metabolism
SOX2
Microscopy
Electron
Transmission
REGENERATION
stem cells
Internal medicine
medicine
Organoid
Animals
PHYSIOLOGY
Science & Technology
IN-VITRO EXPANSION
SOXB1 Transcription Factors
SIDE POPULATION
Transplantation
Mice
Inbred C57BL
030104 developmental biology
SOXB1 Transcription Factors -- genetics -- metabolism
Organoids -- cytology -- metabolism -- ultrastructure
030217 neurology & neurosurgery
Stem Cell Transplantation
Zdroj: Journal of Endocrinology, 240 (2
Journal of Endocrinology
Popis: The pituitary is the master endocrine gland, harboring stem cells of which the phenotype and role remain poorly characterized. Here, we established organoids from mouse pituitary with the aim to generate a novel research model to study pituitary stem cell biology. The organoids originated from the pituitary cells expressing the stem cell marker SOX2, were long-term expandable, displayed a stemness phenotype during expansive culture and showed specific hormonal differentiation ability, although limited, after subrenal transplantation. Application of the protocol to transgenically injured pituitary harboring an activated stem cell population, resulted in more numerous organoids. Intriguingly, these organoids presented with a cystic morphology whereas the organoids from undamaged gland were predominantly dense, and appeared more limited in expandability. Transcriptomic analysis revealed distinct epithelial phenotypes and showed that cystic organoids more resembled the pituitary phenotype, at least to an immature state, and displayed in vitro differentiation, although yet moderate. Organoid characterization further exposed facets of regulatory pathways of the putative stem cells of the pituitary and advanced new injury-activated markers. Taken together, we established a novel organoid research model revealing new insights into the identity and regulation of the putative pituitary stem cells. This organoid model may eventually lead to an interesting tool to decipher pituitary stem cell biology in both healthy and diseased gland.
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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