Nonenzymatic glycation of fibronectin impairs adhesive and proliferative properties of human vascular smooth muscle cells
| Autor: | Mariella Trovati, S. Burzacca, Isabella Russo, E. Mularoni, Luigi Mattiello, Paola Massucco, Giovanni Anfossi, Franco Cavalot, A.W.A. Hahn |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Glycosylation Vascular smooth muscle biology Chemistry Endocrinology Diabetes and Metabolism Adhesion In vitro Muscle Smooth Vascular Fibronectins Extracellular matrix Fibronectin chemistry.chemical_compound Endocrinology Glycation Internal medicine medicine biology.protein Cell Adhesion Humans Cell adhesion Cell Division Cells Cultured |
| Zdroj: | Metabolism: clinical and experimental. 45(3) |
| ISSN: | 0026-0495 |
| Popis: | Nonenzymatic glycation of proteins is involved in the pathogenesis of diabetes vascular complications. Extracellular matrix proteins are a prominent target for nonenzymatic glycation because of their slow turnover rates. The aim of this study was to investigate the influence of human fibronectin (F) nonenzymatic glycation on adhesion and proliferation of cultured human vascular smooth muscle cells (hVSMC). Incubation of human F with 500 mmol/L D-glucose at 37 degrees C induced a time-dependent increase in fluorescence detectable at 440 nm after excitation at 363 nm. Nonenzymatic glycation did not affect binding of F itself to the plates. Adhesion of hVSMC to F increased with the increase of incubation time of the cells on the protein from 30 minutes up to 120 minutes and remained stable thereafter. Adhesion to glycated fibronectin (GF) was reduced in comparison to control F at all the different adhesion times. Adhesion of hVSMC to GF was reduced when F was exposed to glucose for 4, 9, or 28 days (P=.0417 to .0025), but not when F was exposed for 1 day. Adhesion of hVSMC to GF was reduced compared with adhesion to nonglycated F at all coating concentrations from 0.2 to 10 micrograms/mL (P=.05 to .014). Thus, nonenzymatic glycation of F impairs adhesion of hVSMC in vitro. Proliferation of hVSMC on F increased with increasing concentrations of the protein as coating agent (ANOVA:P |
| Databáze: | OpenAIRE |
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