A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of cystatin C and β2-microglobulin in urine
| Autor: | Ning Tan, Kun Wang, Deng-Xuan Wu, T T Cui, Yuan-hui Liu, Yong Liu, Shi-qun Chen, Wei-jie Bei, Hua-long Li, J Y Chen |
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| Rok vydání: | 2017 |
| Předmět: |
Microbiology (medical)
Chromatography biology Chemistry Beta-2 microglobulin Biochemistry (medical) Clinical Biochemistry Immunology Contrast-induced nephropathy Renal function Urine 030204 cardiovascular system & hematology Fluorescence immunoassay Time resolved fluorescence immunoassay medicine.disease Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Cystatin C 030220 oncology & carcinogenesis medicine biology.protein Immunology and Allergy Competitive immunoassay |
| Zdroj: | British Journal of Biomedical Science. 74:193-197 |
| ISSN: | 0967-4845 |
| DOI: | 10.1080/09674845.2017.1334740 |
| Popis: | Background: Contrast media is widely used in clinical diagnostic and interventional procedures, but may cause damage to the kidney, that is, contrast-induced nephropathy. This study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of renal function markers cystatin-C (Cys-C) and β2-microglobulin (β2-MG) for the early diagnosis and follow-up surveillance of contrast-induced nephropathy.Methods: A sandwich immunoassay was used to detect the concentration of Cys-C, and the competitive immunoassay was used to detect the concentration of β2-MG in 50 samples of urine. The performance of this dual-label TRFIA was evaluated and compared with commercial assays.Results: The sensitivity for Cys-C detection was 1.26 ng/ml, the average recovery was 99.36%; The sensitivity for β2-MG detection was 2.13 ng/ml, the average recovery was 100.18%. Bland–Altman analysis showed that the dual-label TRFIA method and the commercial kits had a good agreement, s... |
| Databáze: | OpenAIRE |
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