Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice
Autor: | Alireza Abdollahi, Tayebeh Mahmoudi, Behrouz Nikbin, Seyed Mahmoud Hashemi, Lobat Tayebi, Shahrzad Nojehdehi, Farshid Noorbakhsh, Kamal Abdolmohammadi, Masoud Soleimani, Mohammad Hossein Nicknam |
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Rok vydání: | 2020 |
Předmět: |
medicine.medical_specialty
medicine.medical_treatment Intraperitoneal injection Pharmaceutical Science Adipose tissue Preconditioning Cerulein 03 medical and health sciences Acute Pancreatitis 0302 clinical medicine In vivo Internal medicine medicine Conditioned Medium General Pharmacology Toxicology and Pharmaceutics 030304 developmental biology Inflammation 0303 health sciences biology business.industry lcsh:RM1-950 Mesenchymal stem cell Hypoxia (medical) medicine.disease lcsh:Therapeutics. Pharmacology Mesenchymal Stem Cell medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis Myeloperoxidase biology.protein Acute pancreatitis medicine.symptom Pancreas business Research Article |
Zdroj: | Advanced Pharmaceutical Bulletin Advanced Pharmaceutical Bulletin, Vol 10, Iss 2, Pp 297-306 (2020) |
ISSN: | 2251-7308 2228-5881 |
Popis: | Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice. Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μg/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 µL/mice/30 min × 3 times). Twelve hours following the treatment, serum levels of amylase and lipase were measured. In addition, pancreas pathological changes, immunohistochemical examinations for evaluation of IL-6 expression and pancreatic myeloperoxidase (MPO) enzyme activity were analyzed. Results: The in vitro results of the morphological, differentiation and immunophenotyping analyses confirmed that hypoxia preconditioned MSCs (HP-MSCs) conserve MSCs characteristics after preconditioning. However, HP-MSCs significantly expressed high mRNA level of hypoxia inducible factor 1-α and higher level of total protein. The in vivo findings of the current study showed that CM and HCM significantly reduced the amylase & lipase activity, the severity of pancreas tissue injury and the expression of IL-6 and MPO enzyme activity compared with the AP group. However, no significant difference between CM and HCM groups was demonstrated. Conclusion: Use of CM and HCM can attenuate cerulein-induced AP and decrease inflammation in the pancreas tissue in AP mice. |
Databáze: | OpenAIRE |
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