A sensitive method to extract DNA from biological traces present on ammunition for the purpose of genetic profiling
| Autor: | Thirsa Kraaijenbrink, Sofia Zuniga, Peter de Knijff, René H.P. Mieremet, Patrick Dieltjes, Jeroen Pijpe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Firearms
Genotype Computational biology Biology Polymerase Chain Reaction Pathology and Forensic Medicine law.invention Forensic dna chemistry.chemical_compound law Technical Note Humans Crime scene Short tandem repeat Cartridge Genotyping Polymerase chain reaction Netherlands Genetics Reproducibility of Results DNA DNA Fingerprinting Casing Biological materials Bullet DNA profiling chemistry Genetic Loci Gun shot residue Microsatellite Crime Microsatellite Repeats |
| Zdroj: | International Journal of Legal Medicine, 125(4), 597-602 International Journal of Legal Medicine |
| DOI: | 10.1007/s00414-010-0454-4 |
| Popis: | Exploring technological limits is a common practice in forensic DNA research. Reliable genetic profiling based on only a few cells isolated from trace material retrieved from a crime scene is nowadays more and more the rule rather than the exception. On many crime scenes, cartridges, bullets, and casings (jointly abbreviated as CBCs) are regularly found, and even after firing, these potentially carry trace amounts of biological material. Since 2003, the Forensic Laboratory for DNA Research is routinely involved in the forensic investigation of CBCs in the Netherlands. Reliable DNA profiles were frequently obtained from CBCs and used to match suspects, victims, or other crime scene-related DNA traces. In this paper, we describe the sensitive method developed by us to extract DNA from CBCs. Using PCR-based genotyping of autosomal short tandem repeats, we were able to obtain reliable and reproducible DNA profiles in 163 out of 616 criminal cases (26.5%) and in 283 out of 4,085 individual CBC items (6.9%) during the period January 2003-December 2009. We discuss practical aspects of the method and the sometimes unexpected effects of using cell lysis buffer on the subsequent investigation of striation patterns on CBCs. |
| Databáze: | OpenAIRE |
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