A nude mouse wilms' tumor line (KCMC-WT-1) derived from an aniridia patient with monoalleleic partial deletion of chromosome 11p
| Autor: | Kiyoshi Imaizumi, Toshiji Nishi, Yohei Miyagi, Ichiro Aoki, Hirokazu Nishihira, Yoji Nagashima, Yoshiro Sasaki, Yukichi Tanaka, Kazuaki Misugi |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology Genes Wilms Tumor Stromal cell Tumor suppressor gene Biopsy Transplantation Heterologous Mice Nude Wilms Tumor Mice Mucoproteins Nude mouse Uromodulin medicine Animals Humans Vimentin Northern blot Aniridia Alleles biology Chromosomes Human Pair 11 Mucin-1 S100 Proteins Cytogenetics Chromosome Mapping Infant Wilms' tumor biology.organism_classification medicine.disease Immunohistochemistry Candidate Tumor Suppressor Gene Kidney Neoplasms Neoplasm Proteins Microscopy Electron Oncology Keratins Chromosome Deletion |
| Zdroj: | Cancer. 77:799-804 |
| ISSN: | 1097-0142 0008-543X |
| DOI: | 10.1002/(sici)1097-0142(19960215)77:4<799::aid-cncr28>3.0.co;2-v |
| Popis: | BACKGROUND. A candidate tumor suppressor gene, WT-1, is believed to have an important role in the pathogenesis of Wilms' tumor, especially that occurring in patients with congenital aniridia. METHODS. To obtain a stable tumor line to work with, Wilms' tumor tissue was serially transplanted in athymic nude mice. Biopsied Wilms' tumor tissue, derived from an aniridia patient, was transplanted subcutaneously to an athymic nude mouse, and then transplanted serially. Histopathologic and molecular biologic studies were performed on the xenotransplants. RESULTS. The aniridia patient showed partial deletion in one short arm of chromosome 11, which bears the WT-1 gene. The tumor was successfully transplanted in the nude mouse. Although the tumor contained blastemic, organoid, and stromal histologic elements, the organoid element began to decrease after more than 20 passages. Cytogenetic analysis revealed an additional abbreviation of one long arm of chromosome 6. Dot blot analysis showed that the copy number of WT-1 gene was decreased to half the amount in the tumor, in spite of the WT-1 transcript with normal size detected by Northern blotting. CONCLUSIONS. The tumor is expected to bear one WT-1 gene with minute abnormalities as well as one congenitally deleted gene. This tumor line is useful when examining the effect caused by introduction of WT-1 gene to Wilms' tumor in vivo. |
| Databáze: | OpenAIRE |
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