Fibronectin-dependent tissue mechanics regulate the translation of segmentation clock oscillations into periodic somite formation
| Autor: | Ana Patrícia Martins-Jesus, Patrícia Gomes de Almeida, Sólveig Thorsteinsdóttir, Gonçalo G. Pinheiro, Pedro Rifes, Raquel P. Andrade |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Chemistry fungi 030302 biochemistry & molecular biology Translation (biology) Matrix (biology) Cell biology Fibronectin CLOCK 03 medical and health sciences Somite medicine.anatomical_structure Somitogenesis medicine biology.protein Paraxial mesoderm Cytoskeleton 030304 developmental biology |
| DOI: | 10.1101/808121 |
| Popis: | Somitogenesis starts with cyclic waves of expression of segmentation clock genes in the presomitic mesoderm (PSM) and culminates with periodic budding of somites in its anterior-most region. How cyclic clock gene expression is translated into timely morphological somite formation has remained unclear. A posterior to anterior gradient of increasing PSM tissue cohesion correlates with increasing fibronectin matrix complexity around the PSM, suggesting that fibronectin-dependent tissue mechanics may be involved in this transition. Here we address whether the mechanical properties of the PSM tissue play a role in regulating the pathway leading to cleft formation in the anterior PSM. We first interfered with cytoskeletal contractility in the chick PSM by disrupting actomyosin-mediated contractility directly or via Rho-associated protein kinase function. Then we perturbed fibronectin matrix accumulation around the PSM tissue by blocking integrin-fibronectin binding or fibronectin matrix assembly. All four treatments perturbedhairy1andmeso1expression dynamics and resulted in defective somitic clefts. A model is presented where a gradient of fibronectin-dependent tissue mechanics participates in the PSM wavefront of maturation by ensuring the correct spatio-temporal conversion of cyclic segmentation clock gene expression into periodic somite formation. |
| Databáze: | OpenAIRE |
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