Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line
| Autor: | Apichai Bourchookarn, Chutima Thepparit, Steven A. Barker, Kevin R. Macaluso, Natthida Petchampai |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
animal structures
Ixodes biology biology.organism_classification Microbiology Virology Rickettsia felis Rickettsiaceae Microbial Pathogenicity Cell Line Histones Bacterial adhesin Rickettsia Histone Ixodes scapularis embryonic structures biology.protein Histone H2B Animals Arachnid Vectors Rickettsiales Bacterial Outer Membrane Proteins Protein Binding |
| Zdroj: | Microbiology |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.041400-0 |
| Popis: | Haematophagous arthropods are the primary vectors in the transmission ofRickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction betweenIxodes scapularishistone H2B andRickettsia felis. Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B withR. felisproteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediatingR. felisinternalization into ISE6 cells. |
| Databáze: | OpenAIRE |
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