GPX1 Localizes to the Nucleus in Prostate Epithelium and its Levels are not Associated with Prostate Cancer Recurrence
| Autor: | Larisa Nonn, Lenny Hong, Virgilia Macias, Alan M Diamond, Dede N. Ekoue, Emmanuel Ansong, Andre Kajdacsy-Balla, Peter H. Gann, Ryan Deaton, Rawan Allozi Rupnow |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
GPX1 Physiology medicine.medical_treatment Clinical Biochemistry Biology Biochemistry Article 03 medical and health sciences Prostate cancer 0302 clinical medicine Prostate LNCaP medicine glutathione peroxidase selenium Molecular Biology Tissue microarray prostate prostatectomy Prostatectomy lcsh:RM1-950 Cell Biology medicine.disease Epithelium 3. Good health 030104 developmental biology medicine.anatomical_structure lcsh:Therapeutics. Pharmacology Cytoplasm 030220 oncology & carcinogenesis Cancer research |
| Zdroj: | Antioxidants, Vol 7, Iss 11, p 167 (2018) Antioxidants Volume 7 Issue 11 |
| ISSN: | 2076-3921 |
| Popis: | Glutathione peroxidase 1 (GPX1) is an extensively studied selenium-dependent protein that reduces hydrogen and lipid peroxides to water. Because of its antioxidant function and its responsiveness to dietary intakes of selenium, an essential trace element whose levels are inversely associated with prostate cancer risk, GPX1 levels were assessed in a prostate cancer tissue microarray, comparing cases of recurrent prostate cancer following prostatectomy to non-recurrent controls. While GPX1 is generally considered as a protein that resides in both the cytoplasm and mitochondria, we detected strong nuclear staining by immunofluorescence using GPX1-specific antibodies. Nuclear localization of GPX1 was also observed in both primary prostate epithelial cells and the immortalized prostate-derived cell line RWPE-1, but not in LNCaP or PC3 prostate tumor-derived cell lines. Quantification of GPX1 levels in the entire cell, the cytoplasm, and the nucleus did not indicate any association of either its levels or subcellular distribution with prostate cancer recurrence. While GPX1 levels may not have an impact on survival among men with prostate cancer, the data indicates that this extensively characterized protein may have a novel function in the nucleus of prostate epithelial cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|