The activation of sea urchin eggs by the divalent ionophores A23187 and X-537A
| Autor: | Berton C. Pressman, Birgit Rose, Edward L. Chambers |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Male
Carboxylic Acids Biophysics Ionophore Biology Cleavage (embryo) Benzoates Biochemistry Membrane Potentials Divalent Human fertilization biology.animal Botany Animals Magnesium Seawater Molecular Biology Sea urchin Ovum chemistry.chemical_classification Valinomycin Electric Conductivity Biological Transport Cell Biology Parthenogenesis Spermatozoa Anti-Bacterial Agents Membrane chemistry Cytoplasm Fertilization Sea Urchins Potassium Calcium Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 60:126-132 |
| ISSN: | 0006-291X |
| Popis: | The divalent ionophores A23187 and X-537A induce parthenogenesis in sea urchin eggs. This results from their ability to mobilize intracellular Ca 2+ , which is implicated in both artificial parthenogenesis as well as the natural fertilization process. A23187 causes expulsion of cortical granules and elevation of the fertilization membrane within 0.5–9 min followed by an initiation of cell cleavage. The broader spectrum ionophore X-537A is less potent, but the production of cytoplasmic aberrations are more apparent. In contrast to the sperm-activated egg, the initial phase of ionophore induced activation is accompanied either by relatively insignificant changes in membrane resistance, or an increase. |
| Databáze: | OpenAIRE |
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