Light-Driven CO2 Reduction by Co-Cytochrome b562
| Autor: | Dayn J. Sommer, Noah Gwerder, Giovanna Ghirlanda, Nicholas Halloran, Rafael Alcala-Torano |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cytochrome
chemistry.chemical_element 010402 general chemistry proton reduction 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry Catalysis chemistry.chemical_compound protein design lcsh:QH301-705.5 Molecular Biology catalysis biology 010405 organic chemistry Carbon fixation Rational design Active site Directed evolution Combinatorial chemistry Porphyrin 0104 chemical sciences lcsh:Biology (General) chemistry CO2 reduction 13. Climate action biology.protein Cobalt cobalt porphyrin |
| Zdroj: | Frontiers in Molecular Biosciences, Vol 8 (2021) |
| ISSN: | 2296-889X |
| DOI: | 10.3389/fmolb.2021.609654 |
| Popis: | The current trend in atmospheric carbon dioxide concentrations is causing increasing concerns for its environmental impacts, and spurring the developments of sustainable methods to reduce CO2 to usable molecules. We report the light-driven CO2 reduction in water in mild conditions by artificial protein catalysts based on cytochrome b562 and incorporating cobalt protoporphyrin IX as cofactor. Incorporation into the protein scaffolds enhances the intrinsic reactivity of the cobalt porphyrin toward proton reduction and CO generation. Mutations around the binding site modulate the activity of the enzyme, pointing to the possibility of further improving catalytic activity through rational design or directed evolution. |
| Databáze: | OpenAIRE |
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