Effect of immunosuppressive drugs on cytokine production in canine whole blood stimulated with lipopolysaccharide or a combination of ionomycin and phorbol 12‐myristate 13‐acetate

Autor: Lakshmi Narayanan, Simon M. Firestone, Todd M. Archer, Caroline S Mansfield, Julien R.S. Dandrieux
Rok vydání: 2019
Předmět:
Lipopolysaccharides
medicine.medical_treatment
Stimulation
Pharmacology
Canine
chemistry.chemical_compound
0302 clinical medicine
Azathioprine
Interferon gamma
immunosuppressive drug
Whole blood
0303 health sciences
Ionomycin
3. Good health
Azathioprine/pharmacology
Immunosuppressive drug
Cytokine
Cyclosporine
Tetradecanoylphorbol Acetate
Original Article
whole blood stimulation
Tetradecanoylphorbol Acetate/toxicity
Immunosuppressive Agents
Leflunomide
medicine.drug
immune monitoring
Tumor Necrosis Factor-alpha/biosynthesis
Prednisone/pharmacology
Mycophenolic acid
Interferon-gamma
03 medical and health sciences
Dogs
Interferon-gamma/biosynthesis
medicine
Animals
030304 developmental biology
General Veterinary
Tumor Necrosis Factor-alpha
Immunosuppressive Agents/pharmacology
business.industry
Interleukins
Ionomycin/toxicity
Original Articles
Mycophenolic Acid
Ciclosporin
cytokines
Interleukins/biosynthesis
Mycophenolic Acid/pharmacology
Lipopolysaccharides/toxicity
Cyclosporine/pharmacology
chemistry
Prednisone
business
Leflunomide/pharmacology
030215 immunology
Zdroj: Dandrieux, J R S, Narayanan, L, Firestone, S, Archer, T M & Mansfield, C S 2019, ' Effect of immunosuppressive drugs on cytokine production in canine whole blood stimulated with lipopolysaccharide or a combination of ionomycin and phorbol 12-myristate 13-acetate ', Veterinary Medicine and Science, vol. 5, no. 2, pp. 199-205 . https://doi.org/10.1002/vms3.143
Veterinary Medicine and Science
ISSN: 2053-1095
DOI: 10.1002/vms3.143
Popis: A pharmacodynamic assay has been previously developed to monitor ciclosporin treatment in dogs by assessing inhibition of cytokine transcription after whole blood stimulation with 12‐myristate 13‐1 acetate and ionomycin (PMA/I). In this study, whole blood stimulation with either PMA/I or lipopolysaccharide (LPS) was used to assess the effect of multiple drugs (azathioprine, ciclosporin, mycophenolate, leflunomide and prednisone) after a 7‐day treatment course on production of cytokines measured with a multiplex assay in healthy dogs (n = 4 for each treatment). Interleukin‐10 (IL‐10), interferon gamma (IFN γ) and tumour necrosis factor alpha (TNF α) were significantly activated by PMA/I stimulation and IL‐6, IL‐10 and TNF α by LPS stimulation, in the absence of immunosuppressive drugs. After ciclosporin treatment, IL‐10, IFN γ and TNF α production was significantly reduced after stimulation with PMA/I compared to pre‐treatment. After prednisone treatment, TNF α production was significantly reduced after stimulation with PMA/I or LPS compared to pre‐treatment. No significant change was observed after treatment with azathioprine, leflunomide or mycophenolate. This methodology may be useful to monitor dogs not only treated with ciclosporin, but also with prednisone or a combination of both. Further studies are needed to assess the use of this assay in a clinical setting.
Databáze: OpenAIRE
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