Bioaccumulation Efficiency, Tissue Distribution, and Environmental Occurrence of Hepatitis E Virus in Bivalve Shellfish from France
| Autor: | Marco Grodzki, Jean-Claude Le Saux, Jacques Le Pendu, Julien Schaeffer, Françoise S. Le Guyader, Jean-Come Piquet, Joanna Ollivier, Julien Chevé |
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| Rok vydání: | 2014 |
| Předmět: |
animal structures
Swine viruses Zoology Food Contamination Real-Time Polymerase Chain Reaction medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Hepatitis E virus Environmental Microbiology medicine Animals 14. Life underwater Ostrea edulis Shellfish 030304 developmental biology 0303 health sciences Ecology biology 030306 microbiology fungi food and beverages Bivalvia biology.organism_classification Hepatitis E medicine.disease Ostreidae 6. Clean water Bioaccumulation Food Microbiology RNA Viral Crassostrea France Food Science Biotechnology |
| Zdroj: | Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2014-07, Vol. 80, N. 14, P. 4269-4276 |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Hepatitis E virus (HEV), an enteric pathogen of both humans and animals, is excreted by infected individuals and is therefore present in wastewaters and coastal waters. As bivalve molluscan shellfish are known to concentrate viral particles during the process of filter feeding, they may accumulate this virus. The bioaccumulation efficiencies of oysters ( Crassostrea gigas ), flat oysters ( Ostrea edulis ), mussels ( Mytilus edulis ), and clams ( Ruditapes philippinarum ) were compared at different time points during the year. Tissue distribution analysis showed that most of the viruses were concentrated in the digestive tissues of the four species. Mussels and clams were found to be more sensitive to sporadic contamination events, as demonstrated by rapid bioaccumulation in less than 1 h compared to species of oysters. For oysters, concentrations increased during the 24-h bioaccumulation period. Additionally, to evaluate environmental occurrence of HEV in shellfish, an environmental investigation was undertaken at sites potentially impacted by pigs, wild boars, and human waste. Of the 286 samples collected, none were contaminated with hepatitis E virus, despite evidence that this virus is circulating in some French areas. It is possible that the number of hepatitis E viral particles discharged into the environment is too low to detect or that the virus may have a very short period of persistence in pig manure and human waste. |
| Databáze: | OpenAIRE |
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