Glycocalyx Degradation Induces a Proinflammatory Phenotype and Increased Leukocyte Adhesion in Cultured Endothelial Cells under Flow
| Autor: | Lisa Danielzak, Scott Cooper, Richard L. Leask, Karli K. McDonald |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Culture Techniques lcsh:Medicine 030204 cardiovascular system & hematology Mechanotransduction Cellular Epithelium White Blood Cells 0302 clinical medicine Animal Cells Leukocytes Medicine and Health Sciences Electron Microscopy Mechanotransduction lcsh:Science Microscopy Multidisciplinary Hydrolysis NF-kappa B Light Microscopy Adhesion Intercellular Adhesion Molecule-1 Biomechanical Phenomena Cell biology Phenotype medicine.anatomical_structure Biological Cultures Scanning Electron Microscopy Cellular Types Anatomy medicine.symptom Rheology Research Article Nitric Oxide Synthase Type III Endothelium Immune Cells Immunology Inflammation Leukocyte Adhesion Biology Glycocalyx Research and Analysis Methods Cell Line Proinflammatory cytokine 03 medical and health sciences Cell Line Tumor Cell Adhesion medicine Humans Cell adhesion Blood Cells lcsh:R Endothelial Cells Biology and Life Sciences Epithelial Cells Cell Biology Cell Cultures Coculture Techniques Biological Tissue 030104 developmental biology Gene Expression Regulation Cardiovascular Anatomy lcsh:Q Stress Mechanical |
| Zdroj: | PLoS ONE, Vol 11, Iss 12, p e0167576 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Leukocyte adhesion to the endothelium is an early step in the pathogenesis of atherosclerosis. Effective adhesion requires the binding of leukocytes to their cognate receptors on the surface of endothelial cells. The glycocalyx covers the surface of endothelial cells and is important in the mechanotransduction of shear stress. This study aimed to identify the molecular mechanisms underlying the role of the glycocalyx in leukocyte adhesion under flow. We performed experiments using 3-D cell culture models, exposing human abdominal aortic endothelial cells to steady laminar shear stress (10 dynes/cm2 for 24 hours). We found that with the enzymatic degradation of the glycocalyx, endothelial cells developed a proinflammatory phenotype when exposed to uniform steady shear stress leading to an increase in leukocyte adhesion. Our results show an up-regulation of ICAM-1 with degradation compared to non-degraded controls (3-fold increase, p |
| Databáze: | OpenAIRE |
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