URINARY PROTEIN EXCRETION OF SEMIDOMESTICATED MINK IN A CHRONIC METHYLMERCURY STUDY
Autor: | N. Larivière, D. Belanger, G. Chamberland, L. Vermette, J. S. Blais, A Dallaire |
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Rok vydání: | 1996 |
Předmět: |
medicine.medical_specialty
Renal glomerulus Urinary system Renal function Food Contamination Urine Kidney Toxicology Excretion Random Allocation chemistry.chemical_compound Internal medicine biology.animal medicine Albuminuria Animals Mink Creatinine Proteinuria biology Chemistry Fishes Transferrin Proteins Methylmercury Compounds Pollution Molecular Weight Endocrinology Immunoglobulin G Electrophoresis Polyacrylamide Gel Female medicine.symptom beta 2-Microglobulin |
Zdroj: | Journal of Toxicology and Environmental Health. 47:285-297 |
ISSN: | 0098-4108 |
DOI: | 10.1080/009841096161799 |
Popis: | A model using semidomesticated mink was set up to study the effects of chronic oral methylmercury exposure in piscivorous mammals. Three groups of mink were fed daily with diets containing approximately 0.1, 0.5, and 0.9 micrograms/g of total mercury. Piscivorous and non-piscivorous fish, naturally contaminated with methylmercury, were used to prepare diets. Renal injury was evaluated using total urine protein/creatinine ratio and differentiation of urinary low-molecular-weight and high-molecular-weight proteins on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The significance of the total urine protein/creatinine ratio data was assessed by comparing the results to a 95% group-based reference interval. The values for total urine protein/creatinine ratio did not reveal any significant increased excretion, and no dose-related trends were observed within the reference interval. Overall the total urine protein data did not suggest renal damage. Analysis of the SDS-PAGE electrophoretograms did not suggest the presence of any persistent glomerular damage in any group. High-molecular-weight proteins were not detected more frequently for any of the dose groups. During the adaptation phase, the B2M-like protein band was not remarked during the visual analysis of the gels. The B2M-like protein band was remarked during the gel analysis only several weeks into the exposure phase. This B2M-like protein band was more prevalent in urine samples taken from minks in the 0.5 and 0.9 micrograms/g groups than in the 0.1 microgram/group. These latter data, however, did not allow an evaluation of a quantitative dose-response excretion with time. The B2M-like data are suggestive of very minor renal injury. |
Databáze: | OpenAIRE |
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