Upstream development of Escherichia coli fermentation process with PhoA promoter using design of experiments (DoE)
| Autor: | Mian Huang, Renija George, Frank K. Agbogbo, Phil Ramsey, Jobin Joy, Shikha Srivastava, Jesse McCool |
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| Rok vydání: | 2020 |
| Předmět: |
Bioengineering
medicine.disease_cause Applied Microbiology and Biotechnology law.invention Phosphates chemistry.chemical_compound law medicine Escherichia coli Food science Promoter Regions Genetic Strain (chemistry) PhoA E. coli Phosphate Titer Minimal effect chemistry Fermentation Cell Culture and Bioengineering - Original Paper DoE Fermentation Recombinant DNA Biotechnology Model |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1476-5535 |
| Popis: | In this work, a fed-batch fermentation development was performed with recombinant E. coli carrying the PhoA promoter system. The phosphate concentrations tested for this PhoA strain, 2.79 mM to 86.4 mM, were beyond the concentrations previously evaluated for cell growth and product titer. The results from the scouting work was used for design of experiments (DoE) where a range of phosphate levels from 27.1 mM to 86.4 mM was simultaneously evaluated with temperature, pH and DO set points. Definitive screening was used to evaluate these parameters simultaneously and the results indicate that fermentation temperature and phosphate content are the major contributors of product titer. The other factors tested such as pH had a minimal effect and DO had no impact on product titer. Electronic supplementary material The online version of this article (10.1007/s10295-020-02302-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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