Simultaneous determination of total cholesterol concentration and radioactivity in plasma
Autor: | T.W. Fenton, B. V. Turner, B. Emmanuel, L. P. Milligan |
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Rok vydání: | 1983 |
Předmět: |
Chromatography
Chromatography Gas Chemistry Cholesterol Liquid scintillation counting Biophysics Plasma Acetates Biochemistry law.invention Rats chemistry.chemical_compound Liver law Total cholesterol Blood plasma Flame ionization detector Animals lipids (amino acids peptides and proteins) Gas chromatography Propionates Effluent |
Zdroj: | Journal of biochemical and biophysical methods. 8(1) |
ISSN: | 0165-022X |
Popis: | Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific. |
Databáze: | OpenAIRE |
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