Simultaneous determination of total cholesterol concentration and radioactivity in plasma

Autor: T.W. Fenton, B. V. Turner, B. Emmanuel, L. P. Milligan
Rok vydání: 1983
Předmět:
Zdroj: Journal of biochemical and biophysical methods. 8(1)
ISSN: 0165-022X
Popis: Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific.
Databáze: OpenAIRE